ArticleScientific reports2019
A mutation in the AdhE alcohol dehydrogenase of Clostridium thermocellum increases tolerance to several primary alcohols, including isobutanol, n-butanol and ethanol.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 57 citations in OpenAlex.
- Effects of heterologous expression of glycolysis enzymes on product formation inMetabolic engineering communications · 2026Article
- Genetic and metabolic drivers of membrane remodeling inmSystems · 2026Article
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- The role of AdhE mutations inJournal of bacteriology · 2025Article
- Pyrophosphate-free glycolysis in Clostridium thermocellum increases both thermodynamic driving force and ethanol titers.Biotechnology for biofuels and bioproducts · 2024Article
- Expression and characterization of monofunctional alcohol dehydrogenase enzymes inMetabolic engineering communications · 2024Article
- The role of AdhE on ethanol tolerance and production in Clostridium thermocellum.The Journal of biological chemistry · 2024Article
- The potential of native and engineered Clostridia for biomass biorefining.Frontiers in bioengineering and biotechnology · 2024Review
- Ethanol tolerance in engineered strains of Clostridium thermocellum.Biotechnology for biofuels and bioproducts · 2023Article
- Current progress on engineering microbial strains and consortia for production of cellulosic butanol through consolidated bioprocessing.Microbial biotechnology · 2023Review
- Ethanol tolerance of Clostridium thermocellum: the role of chaotropicity, temperature and pathway thermodynamics on growth and fermentative capacity.Microbial cell factories · 2022Article
- Increasing the Thermodynamic Driving Force of the Phosphofructokinase Reaction inApplied and environmental microbiology · 2022Article
- Increasing Solvent Tolerance to Improve Microbial Production of Alcohols, Terpenoids and Aromatics.Microorganisms · 2021Review
- Article
- Ethanol tolerance assessment in recombinant E. coli of ethanol responsive genes from Lactobacillus buchneri NRRL B-30929.World journal of microbiology & biotechnology · 2020Article
- High-resolution structure of the alcohol dehydrogenase domain of the bifunctional bacterial enzyme AdhE.Acta crystallographica. Section F, Structural biology communications · 2020Article
- Identification of 40S ribosomal protein S8 as a novel biomarker for alcohol‑associated hepatocellular carcinoma using weighted gene co‑expression network analysis.Oncology reports · 2020Observational
- Consolidated bioprocessing for butanol production of cellulolytic Clostridia: development and optimization.Microbial biotechnology · 2020Review
- Pathway dissection, regulation, engineering and application: lessons learned from biobutanol production by solventogenic clostridia.Biotechnology for biofuels · 2020Review
- Modeling ethanol production through gas fermentation: a biothermodynamics and mass transfer-based hybrid model for microbial growth in a large-scale bubble column bioreactor.Biotechnology for biofuels · 2020Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Clostridium thermocellum is a good candidate organism for producing cellulosic biofuels due to its native ability to ferment cellulose, however its maximum biofuel titer is limited by tolerance. Wild type C. thermocellum is inhibited by 5 g/L n-butanol. Using growth adaptation in a chemostat, we increased n-butanol tolerance to 15 g/L. We discovered that several tolerant strains had acquired a D494G mutation in the adhE gene. Re-introducing this mutation recapitulated the n-butanol tolerance phenotype. In addition, it increased tolerance to several other primary alcohols including isobutanol and ethanol. To confirm that adhE is the cause of inhibition by primary alcohols, we showed that deleting adhE also increases tolerance to several primary alcohols.
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