ArticleDevelopmental dynamics : an official publication of the American Association of Anatomists2019
Aggregation, segregation, and dispersal of homotypic germ plasm RNPs in the early zebrafish embryo.
Article in Developmental dynamics : an official publication of the American Association of Anatomists, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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27 citing papers in PubMed, 39 citations in OpenAlex.
- Discrete 3' UTR sequence motifs regulate nanos RNA enrichment in Drosophila germ granules.Developmental biology · 2026Article
- How do RNA molecules distinguish self from non-self?Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Germ granule-mediated mRNA storage and translational control.RNA biology · 2025Review
- Controlling intermolecular base pairing in Drosophila germ granules by mRNA folding and its implications in fly development.Nature communications · 2025Article
- Novel structure and composition of the unusually large germline determinant of the wasp Nasonia vitripennis.Molecular biology of the cell · 2025Article
- The piRNA protein Asz1 is essential for germ cell and gonad development in zebrafish and exhibits differential necessities in distinct types of germ granules.PLoS genetics · 2025Article
- Novel structure and composition of the unusually large germline determinant of the wasp Nasonia vitripennis.bioRxiv : the preprint server for biology · 2024Article
- Germ plasm dynamics during oogenesis and early embryonic development in Xenopus and zebrafish.Molecular reproduction and development · 2024Review
- Caenorhabditis elegans germ granules accumulate hundreds of low translation mRNAs with no systematic preference for germ cell fate regulators.Development (Cambridge, England) · 2024Article
- Spatial organization and function of RNA molecules within phase-separated condensates in zebrafish are controlled by Dnd1.Developmental cell · 2023Article
- Germ Granule Evolution Provides Mechanistic Insight into Drosophila Germline Development.Molecular biology and evolution · 2023Article
- Localization of oskar mRNA by agglomeration in ribonucleoprotein granules.PLoS genetics · 2023Article
- Modern tools applied to classic structures: Approaches for mammalian male germ cell RNA granule research.Andrology · 2023Review
- Germ granules in development.Development (Cambridge, England) · 2023Article
- Structural and functional organization of germ plasm condensates.The Biochemical journal · 2022Review
- Germ Granules in Animal Oogenesis.Journal of developmental biology · 2022Review
- Sinhcaf-dependent histone deacetylation is essential for primordial germ cell specification.EMBO reports · 2022Article
- Computational modeling offers new insight into Drosophila germ granule development.Biophysical journal · 2022Article
- Determining the Role of Maternally-Expressed Genes in Early Development with Maternal Crispants.Journal of visualized experiments : JoVE · 2021Article
- Conserved germ plasm characteristics across the Danio and Devario lineages.Genesis (New York, N.Y. : 2000) · 2021Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
backgroundIn zebrafish and many other organisms, specification of primordial germ cells (PGCs) requires the transmission of maternally-derived germ plasm. Zebrafish germ plasm ribonucleoparticles (RNPs) aggregate along the cleavage furrows during the first several cell cycles, segregate asymmetrically during the cleavage stages, and undergo cytoplasmic dispersal in the late blastula.
resultsFor all tested germ plasm RNAs [carbonic anhydrase 15b (ca15b), deleted in azoospermia-like (dazl), dead end (dnd), nanos 3 (nos3), regulator of G-protein signaling14a (rgs14a), and vasa/DEAD box polypeptide 4 (vasa/ddx4)], RNPs are homotypic (containing a single RNA type), with RNPs packing tightly yet remaining distinct within germ plasm aggregates. Homotypic clustering of RNAs within RNPs is observed before aggregation in the cortex and is maintained through germ plasm recruitment, asymmetric segregation and RNP dispersal. We also identify a step of germ plasm fragmentation during the cleavage stages that precedes RNP dispersal.
conclusionsOur findings suggest that germ plasm aggregates act as subcellular compartments that temporarily collect and carry single RNA-type RNPs from fertilization until their cytoplasmic dispersal in PGCs at the end of the blastula period, and describe a previously unknown fragmentation step that allows for an increase in the pool of germ plasm-carrying cells, presumably PGCs. Developmental Dynamics 248:306-318, 2019. © 2019 Wiley Periodicals, Inc.
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