Evidence map›Paper›PMID 30741345›Full record

ArticleMikrochimica acta2019

An impedimetric biosensor for E. coli O157:H7 based on the use of self-assembled gold nanoparticles and protein G.

Donghai Lin, Rajesh G Pillai, William Edward Lee, Abebaw B Jemere

Abstract read
PubMed Publisher
In one paragraph

Article in Mikrochimica acta, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Thiolated Nanoparticles for Biomedical Applications: Mimicking the Workhorses of Our Body.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022
    Review
  8. Review
  9. Review
  10. Review
  11. Review
  12. Article
  13. Electrochemical impedimetric biosensors for food safety.Food science and biotechnology · 2020
    Review
  14. Article
  15. Article
  16. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Donghai LinDepartment of Chemistry, University of Alberta, Edmonton, AB, T6G 2G2, Canada.
Rajesh G PillaiNational Research Council Canada - Nanotechnology Research Centre (Formerly known as National Institute for Nanotechnology), 11421 Saskatchewan Drive, Edmonton, AB, T6G 2M9, Canada.
William Edward LeeDefence Research and Development Canada - Suffield Research Centre, Medicine Hat, AB, T1A 8K6, Canada.
Abebaw B JemereNational Research Council Canada - Nanotechnology Research Centre (Formerly known as National Institute for Nanotechnology), 11421 Saskatchewan Drive, Edmonton, AB, T6G 2M9, Canada. abebaw.jemere@nrc-cnrc.gc.ca.ORCID http://orcid.org/0000-0002-8205-7304

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Two kinds of electrochemical impedimetric biosensors for the detection of E. coli O157:H7 are described and compared. They were fabricated using self-assembled layers of thiolated protein G (PrG-thiol) on (i) planar gold electrodes and (ii) gold nanoparticles (Au NPs) modified gold electrodes. The fabrications of the biosensors were characterized using cyclic voltammetry, electrochemical impedance spectroscopy, scanning electron microscopy and atomic force microscopy techniques. The modification of the planar gold electrode by Au NPs via self-assembled monolayer of 1,6-hexadithiol as a linker molecule increased the electrochemically active surface area by about 2.2 times. The concentration of PrG-thiol and its incubation time, as well as the concentration of IgG were optimized. The Au NP-based biosensor exhibited a limit of detection of 48 colony forming unit (cfu mL

Indexed as

Biosensing TechniquesDielectric SpectroscopyElectrochemical TechniquesElectrodesEscherichia coli O157Escherichia coli ProteinsGoldMetal NanoparticlesEscherichia coli ProteinsGoldBiosensorE. ColiElectrochemical impedance spectroscopyGold nanoparticlesOriented antibody immobilizationPathogen detection

Identifiers

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.