ArticleMolecular ecology resources2019
SymPortal: A novel analytical framework and platform for coral algal symbiont next-generation sequencing ITS2 profiling.
Article in Molecular ecology resources, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 128 papers.
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Who cites it
128 citing papers in PubMed, 319 citations in OpenAlex.
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- Coral-associated microbiome dynamics under thermal and pollution stress.Conservation biology : the journal of the Society for Conservation Biology · 2026Article
- Assisted evolution of corals and their symbionts enhances recruit heat tolerance but with complex outcomes.Science advances · 2026Article
- Biogeographical Patterns of Coral-Associated Symbiodiniaceae Across Thermal Regimes Including Trace Molecular Detection ofEcology and evolution · 2026Article
- Seasonal Physiological Strategies Reveal Contrasting Host-Symbiont Dynamics Among Dominant Indo-Pacific Reef-Building Corals.Ecology and evolution · 2026Article
- Heatwave winners and losers: cryptic coral holobionts differ in thermal tolerance.Proceedings. Biological sciences · 2026Article
- Stress-Resistant Symbiodiniaceae and Diverse Bacterial Communities Promote Coral Persistence in Variable, Multi-Stressor Environments.Ecology and evolution · 2026Article
- Symbiosis collapses during development of asexual offspring in the absence of heterotrophic feeding in a model cnidarian-algal symbiosis.Scientific reports · 2026Article
- Coral color morphs exhibit distinct microbial and proteomic profiles linked to stress and immune mechanisms in a changing ocean.Microbiome · 2026Article
- Persistent Legacy Effects of Marine Heatwaves on Coral Symbioses.Global change biology · 2026Article
- Metaproteome Analysis of Short-Term Thermal Stress in Three Sympatric Coral Species Reveals Divergent Host Responses.Ecology and evolution · 2026Article
- Differential Responses to Heat Stress Between Freshly Isolated and Long-Term CulturedMicroorganisms · 2026Article
- Rapid Evolution in a Coral Population Following a Mass Mortality Event.Evolutionary applications · 2026Article
- Genome Skimming Illuminates Hidden Species Diversity and Symbiodiniaceae Associations in East Pacific Pocillopora Corals.Genome biology and evolution · 2026Article
- Strong Symbiodiniaceae Influence on Coral Gene Expression Under Ocean Acidification and Warming.Integrative and comparative biology · 2026Article
- Symbiodiniaceae Community Structure and Thermal Tolerance in Soft Corals from Captive Aquarium Environments.Integrative and comparative biology · 2026Article
- Convergent symbioses: morphology, life history, and niche specialization in coral and lichen mutualisms.FEMS microbiology reviews · 2026Review
- Subtropical specialists dominate a coral range expansion front.Coral reefs (Online) · 2026Article
68 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors at 5 institutions in 3 countries.
Funding
Abstract
We present SymPortal (SymPortal.org), a novel analytical framework and platform for genetically resolving the algal symbionts of reef corals using next-generation sequencing (NGS) data of the ITS2 rDNA. Although the ITS2 marker is widely used to genetically characterize taxa within the family Symbiodiniaceae (formerly the genus Symbiodinium), the multicopy nature of the marker complicates its use. Commonly, the intragenomic diversity resultant from this multicopy nature is collapsed by analytical approaches, thereby focusing on only the most abundant sequences. In contrast, SymPortal employs logic to identify within-sample informative intragenomic sequences, which we have termed 'defining intragenomic variants' (DIVs), to identify ITS2-type profiles representative of putative Symbiodiniaceae taxa. By making use of this intragenomic ITS2 diversity, SymPortal is able to resolve genetic delineations using the ITS2 marker at a level that was previously only possible by using additional genetic markers. We demonstrate this by comparing this novel approach to the most commonly used alternative approach for NGS ITS2 data, the 97% similarity clustering to operational taxonomic units (OTUs). The SymPortal platform accepts NGS raw sequencing data as input to provide an easy-to-use, standardization-enforced, and community-driven framework that integrates with a database to gain resolving power with increased use. We consider that SymPortal, in conjunction with ongoing large-scale sampling and sequencing efforts, should play an instrumental role in making future sampling efforts more comparable and in maximizing their efficacy in working towards the classification of the global Symbiodiniaceae diversity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.