ArticleJournal of anatomy2019
Bioengineering the microanatomy of human skin.
Article in Journal of anatomy, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
71 citing papers in PubMed.
- Biometric Quantification of Keratinocyte Turnover Using Bioengineered Human Skin Equivalents.Methods in molecular biology (Clifton, N.J.) · 2027Article
- Visualization and Quantification of Basal Melanocytes Within a 3D Bioengineered Skin Equivalent.Methods in molecular biology (Clifton, N.J.) · 2027Article
- Decoding melanin dynamics: Photoacoustic microscopy reveals differential aging of metabolic and keratinized skin zones.Photoacoustics · 2026Article
- HPMA polymers as functional excipients in dermal nanoformulations of imiquimod.International journal of pharmaceutics: X · 2026Article
- Article
- The influence of skin physicochemical properties on electrical sensory sensitivity during transcutaneous electrical nerve stimulation in women.Scientific reports · 2026Article
- Effects of proton pump inhibitors on remodeling and fibrosis in eosinophilic esophagitis.The Journal of allergy and clinical immunology · 2026Article
- Cellular and molecular changes in the skin driving increased nociception and pain during burn injury and repair.Frontiers in pain research (Lausanne, Switzerland) · 2026Review
- Anatomy of a bioengineered human pigmented skin equivalent to provide fundamental insights into skin tone melanin dynamics.Journal of anatomy · 2026Article
- A Bioengineered Skin Organoid Platform for Modeling Human Skin Physiology and Cytotoxicity.Research square · 2025Article
- Article
- Elucidation of collagen content in different anatomical regions of the dermis of donkeys (Equus asinus): histomorphometric and ultrastructural study.BMC veterinary research · 2025Article
- Electroactive Electrospun Nanofibrous Scaffolds: Innovative Approaches for Improved Skin Wound Healing.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Therapeutic Effects ofInternational journal of molecular sciences · 2025Article
- Article
- Assessing the impact of airborne particulate pollution on human skin utilizing a novel human skin equivalent containing MUTZ-3-derived Langerhans cells.Bioengineering & translational medicine · 2025Article
- Current application of tissue-engineered dermal scaffolds mimicking the extracellular matrix microenvironment in wound healing.Regenerative therapy · 2025Review
- Advancements in 3D skin bioprinting: processes, bioinks, applications and sensor integration.International journal of extreme manufacturing · 2025Review
- Recombinant Humanized Collagen Enhances Secreted Protein Levels of Fibroblasts and Facilitates Rats' Skin Basement Membrane Reinforcement.Journal of functional biomaterials · 2025Article
- Engineering a Pigmented Skin Equivalent That Is Responsive to External Stimuli.Methods in molecular biology (Clifton, N.J.) · 2025Article
11 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
16 authors.
Funding
Abstract
Recreating the structure of human tissues in the laboratory is valuable for fundamental research, testing interventions, and reducing the use of animals. Critical to the use of such technology is the ability to produce tissue models that accurately reproduce the microanatomy of the native tissue. Current artificial cell-based skin systems lack thorough characterisation, are not representative of human skin, and can show variation. In this study, we have developed a novel full thickness model of human skin comprised of epidermal and dermal compartments. Using an inert porous scaffold, we created a dermal construct using human fibroblasts that secrete their own extracellular matrix proteins, which avoids the use of animal-derived materials. The dermal construct acts as a foundation upon which epidermal keratinocytes were seeded and differentiated into a stratified keratinised epithelium. In-depth morphological analyses of the model demonstrated very close similarities with native human skin. Extensive immunostaining and electron microscopy analysis revealed ultrastructural details such as keratohyalin granules and lamellar bodies within the stratum granulosum, specialised junctional complexes, and the presence of a basal lamina. These features reflect the functional characteristics and barrier properties of the skin equivalent. Robustness and reproducibility of in vitro models are important attributes in experimental practice, and we demonstrate the consistency of the skin construct between different users. In summary, a new model of full thickness human skin has been developed that possesses microanatomical features reminiscent of native tissue. This skin model platform will be of significant interest to scientists researching the structure and function of human skin.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.