Assaying NanoLuc Luciferase Activity from mRNA-Injected Xenopus Embryos.

Michael D Sheets

Open access · greenAbstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact, top 97% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

Cytoplasmic Polyadenylation Is an Ancestral Hallmark of Early Development in Animals.Molecular biology and evolution · 2023
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

1 author at 1 institution in 1 country.

Michael D SheetsDepartment of Biomolecular Chemistry, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI, USA. mdsheets@wisc.edu.

University of Wisconsin–Madison · US

Funding

Regulation of cell fates by the Bicaudal-C translational repressorR01HD091921 · NICHD · UNIVERSITY OF WISCONSIN-MADISON · PI SHEETS, MICHAEL D · 2018 to 2022

$1.5M

Defining the Xenopus translatomeR21HD076828 · NICHD · UNIVERSITY OF WISCONSIN-MADISON · PI SHEETS, MICHAEL D · 2013 to 2014

$393k

NICHD NIH HHS R01 HD091921NICHD NIH HHS R21 HD076828

6 · The paper itself

Abstract

The earliest steps of animal development depend upon posttranscriptional events that drive the embryonic cell cycle and guide cell fate decisions. The analysis of post-transcriptional regulatory events has relied upon the use of chimeric reporter mRNAs that encode firefly luciferase fused to potential regulatory sequences. A new and more sensitive luciferase developed recently called NanoLuc has the potential to improve reporter studies and provide new insights into the regulation of embryonic processes. Here I describe how to create and analyze reporter mRNAs encoding NanoLuc luciferase using extracts from microinjected Xenopus embryos.

Indexed as

Gene ExpressionGenes, ReporterLuminescent MeasurementsMicroinjectionsAnimalsEmbryo, NonmammalianIn Vitro TechniquesLuciferasesPlasmidsRecombinant Fusion ProteinsRNA, MessengerTranscription, GeneticXenopus laevisLuciferasesRecombinant Fusion ProteinsRNA, MessengerLuciferasemRNA microinjectionNanoLucXenopus embryos

Identifiers

PMID30737684

DOI10.1007/978-1-4939-9009-2_3

PMCPMC7216303

OpenAlexW2912006657

What OpenQuestion holds

Textmetadata

LicenceTDM

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.