ArticleScientific reports2019
A new high-resolution melting analysis for the detection and identification of Plasmodium in human and Anopheles vectors of malaria.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 25 citations in OpenAlex.
- Sequence-Dependent PNA-AgNP Bioconjugation Enables Electrochemical Discrimination of a Single-Base Mutation in Zika Virus RNA.ACS omega · 2026Article
- Performance of High-Resolution Melting Analysis for Molecular Detection and Species Differentiation of Malaria: A Scoping Review.Pathogens (Basel, Switzerland) · 2026Article
- Molecular Detection of Dengue and Malaria Parasites in Field-Collected Mosquitoes from Meta, Colombia: Implications for Vector-Borne Disease Surveillance.Epidemiologia (Basel, Switzerland) · 2026Article
- Optimization of the real-time PCR platform method using high-resolution melting analysis and comparison with sequencing and phylogenetic analysis for developing optimal malaria diagnostic methods.Scientific reports · 2025Article
- The toolbox for mosquito vector research.Parasites & vectors · 2025Review
- Advances in Malaria Diagnostic Methods in Resource-Limited Settings: A Systematic Review.Tropical medicine and infectious disease · 2024Review
- Pathogen prospecting of museums: Reconstructing malaria epidemiology.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- A novel sensitive hexaplex high-resolution melt assay for identification of five human Plasmodium species plus internal control.Acta tropica · 2023Article
- A targeted amplicon sequencing panel to simultaneously identify mosquito species and Plasmodium presence across the entire Anopheles genus.Molecular ecology resources · 2022Article
- Article
- A genotyping assay to determine geographic origin and transmission potential of Plasmodium falciparum malaria cases.Communications biology · 2021Article
- Rapid detection of Mycoplasma mycoides subsp. capri and Mycoplasma capricolum subsp. capripneumoniae using high-resolution melting curve analysis.Scientific reports · 2021Article
- Surveillance of Plasmodium falciparum pfcrt haplotypes in southwestern uganda by high-resolution melt analysis.Malaria journal · 2021Article
- Molecular detection of apicomplexan blood parasites of coral reef fishes from free-living stages of ectoparasitic gnathiid isopods.Parasitology research · 2020Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Among vector-borne diseases malaria is the leading cause of morbidity in the world, with more than 200 million cases per year and a large number of deaths. The techniques traditionally used for the detection of Plasmodium in humans and Anopheles mosquitoes include microscopy, IRMA, ELISA, antibody or molecular assays, and anopheline dissection. However, these techniques are limited by their requirement of skilled personnel, low sensitivity or long processing times. A PCR-based high-resolution melting (PCR-HRM) analysis was developed for the detection and identification of P. falciparum, P. vivax and P. malariae that infect humans and Anopheles. In 41 human samples PCR-HRM detected 14 samples positive for P. vivax, 17 for P. falciparum, three for P. malariae, three mixed infections for P. vivax/P. malariae and four negative samples. Whereas benchmarking assays of microscopy and nested PCR had false positive detections. Additionally, PCR-HRM was able to detect natural infection with Plasmodium spp. in An. darlingi and An. mattogrossensis. The PCR-HRM presented is the first single assay developed for the detection and identification of P. vivax, P. falciparum and/or P. malariae in human and Anopheles. This method improves on currently available assays as it is easy-to-use, rapid, sensitive and specific with a low risk of contamination.
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