Evidence map›Paper›PMID 30736943›Full record

ArticleVeterinary parasitology2019

Comparison of multiplexed-tandem real-time PCR panel with reference real-time PCR molecular diagnostic assays for detection of Giardia intestinalis and Tritrichomonas foetus in cats.

Maira N Meggiolaro, Florian Roeber, Victoria Kobylski, Damien P Higgins, Jan Šlapeta

Abstract readComparative StudyEvaluation Study
In one paragraph

Article in Veterinary parasitology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed.

  1. Article
  2. Article
  3. Prevalence ofPathogens (Basel, Switzerland) · 2025
    Article
  4. Article
  5. Article
  6. Article
  7. International journal for parasitology. Parasites and wildlife · 2022
    Article
  8. Article
  9. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Maira N MeggiolaroVeterinary Pathology Diagnostic Services, Sydney School of Veterinary Science, University of Sydney, NSW 2006, Australia.
Florian RoeberAusDiagnostics Pty. Ltd., Mascot, 2020, NSW, Australia.
Victoria KobylskiAusDiagnostics Pty. Ltd., Mascot, 2020, NSW, Australia.
Damien P HigginsVeterinary Pathology Diagnostic Services, Sydney School of Veterinary Science, University of Sydney, NSW 2006, Australia.
Jan ŠlapetaVeterinary Pathology Diagnostic Services, Sydney School of Veterinary Science, University of Sydney, NSW 2006, Australia. Electronic address: jan.slapeta@sydney.edu.au.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Giardia intestinalis and Tritrichomonas foetus are frequent enteric protozoan parasites of the gastrointestinal track of domestic cats. Because of different treatment options for the parasites, confirmation of presence of one or both pathogens is necessary. The PCR based assays are suitable for differential diagnosis. We evaluated the performance of Small Animal Diarrhoea panel, a multiplexed-tandem real-time PCR (MT-PCR) assay, that detects DNA of both G. intestinalis and T. foetus. The sensitivity and specificity were compared to reference real-time PCR assays using 105 faecal samples, 39.05% (n = 41) positive for G. intestinalis and 30.48% (n = 32) were positive for T. foetus. The faecal samples positive for T. foetus had a high proportion of late amplifiers, determined by an arbitrary threshold of C

Indexed as

AnimalsCatsDiarrheaGenotypeGiardia lambliaMultiplex Polymerase Chain ReactionProtozoan Infections, AnimalReagent Kits, DiagnosticReal-Time Polymerase Chain ReactionReproducibility of ResultsSensitivity and SpecificityTritrichomonas foetusReagent Kits, DiagnosticDiagnosticsDiarrhoeaFelineGiardiaGiardiasisMT-PCRTaqManTrichomonosisTritrichomonas

Identifiers

PMID30736943
PMCPMC7115774

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.