ArticleGenes2019
Improved Protocols of ITS1-Based Metabarcoding and Their Application in the Analysis of Plant-Containing Products.
Article in Genes, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Proteogenomic investigation of plant constituents in herbal beverages.NPJ science of food · 2026Article
- A critical review of conventional and emerging technologies for the detection of contaminants, allergens and adulterants in plant-based milk alternatives.Current research in food science · 2025Review
- Three-tiered authentication of herbal traditional Chinese medicine ingredients used in women's health provides progressive qualitative and quantitative insight.Frontiers in pharmacology · 2024Article
- Aerobiological Monitoring and Metabarcoding of Grass Pollen.Plants (Basel, Switzerland) · 2023Article
- Article
- Complementary authentication of Chinese herbal products to treat endometriosis using DNA metabarcoding and HPTLC shows a high level of variability.Frontiers in pharmacology · 2023Article
- A detailed workflow to develop QIIME2-formatted reference databases for taxonomic analysis of DNA metabarcoding data.BMC genomic data · 2022Article
- Advancements and future prospective of DNA barcodes in the herbal drug industry.Frontiers in pharmacology · 2022Review
- Mitochondrial Function as a Potential Tool for Assessing Function, Quality and Adulteration in Medicinal Herbal Teas.Frontiers in pharmacology · 2021Review
- Applied Barcoding: The Practicalities of DNA Testing for Herbals.Plants (Basel, Switzerland) · 2020Article
- PLANiTS: a curated sequence reference dataset for plant ITS DNA metabarcoding.Database : the journal of biological databases and curation · 2020Article
Corrections and comments
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plants are widely used for food and beverage preparation, most often in the form of complex mixtures of dried and ground parts, such as teas, spices or herbal medicines. Quality control of such products is important due to the potential health risks from the presence of unlabelled components or absence of claimed ones. A promising approach to analyse such products is DNA metabarcoding due to its high resolution and sensitivity. However, this method's application in food analysis requires several methodology optimizations in DNA extraction, amplification and library preparation. In this study, we present such optimizations. The most important methodological outcomes are the following: 1) the DNA extraction method greatly influences amplification success; 2) the main problem for the application of metabarcoding is DNA purity, not integrity or quantity; and 3) the "non-amplifiable" samples can be amplified with polymerases resistant to inhibitors. Using this optimized workflow, we analysed a broad set of plant products (teas, spices and herbal remedies) using two NGS platforms. The analysis revealed the problem of both the presence of extraneous components and the absence of labelled ones. Notably, for teas, no correlation was found between the price and either the absence of labelled components or presence of unlabelled ones; for spices, a negative correlation was found between the price and presence of unlabelled components.
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