ArticleJournal of the American Chemical Society2019
Cadasides, Calcium-Dependent Acidic Lipopeptides from the Soil Metagenome That Are Active against Multidrug-Resistant Bacteria.
Article in Journal of the American Chemical Society, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed.
- Reprogramming the enduracidin NRPS assembly line via thioesterase domain relocation.Synthetic and systems biotechnology · 2027Article
- Mechanistic Studies of the Calcium-Dependent Antibiotics via Cofactor Engineering.Journal of natural products · 2026Article
- Article
- Advances in natural product discovery: strategies, technologies, and insights.Natural products and bioprospecting · 2026Review
- Comparative genomics reveals hidden biosynthetic diversity inFrontiers in microbiology · 2026Article
- Discovery and Heterologous Expression of the Soil Metagenome-Derived Lasso Peptide Metanodin with an Unprecedented Ring Structure.Journal of natural products · 2025Article
- Novel Synthetic Strategies Towards Analogues of Cadaside and Malacidin Antibiotic Peptides.Biomolecules · 2025Article
- Integrated Omics-Based Discovery of Bioactive Halogenated Metabolites from the Deep-SeaMarine drugs · 2025Article
- Genome Mining Leads to the Discovery of Kasichelins A-D, Unusual β-Alanine- and β-Aminoisobutyric Acid-Containing Siderophores fromJournal of natural products · 2025Article
- Comparative Analysis of Signature Sequences from Adenylation Domains Situated within Bacterial-Origin Nonribosomal Peptide Synthetase Modules.Journal of microbiology and biotechnology · 2025Article
- Structure-filtered search of enzyme variants.Computational and structural biotechnology journal · 2025Article
- Review
- Analysis of Co-localized Biosynthetic Gene Clusters Identifies a Membrane-Permeabilizing Natural Product.Journal of natural products · 2024Article
- Cinnamosyn, a Cinnamoylated Synthetic-Bioinformatic Natural Product with Cytotoxic Activity.Organic letters · 2024Article
- A Boron-Dependent Antibiotic Derived from a Calcium-Dependent Antibiotic.Angewandte Chemie (International ed. in English) · 2024Article
- Discovery of thiazostatin D/E using UPLC-HR-MS2-based metabolomics and σ-factor engineering ofFrontiers in bioengineering and biotechnology · 2024Article
- Potentiality of Actinomycetia Prevalent in Selected Forest Ecosystems in Assam, India to Combat Multi-Drug-Resistant Microbial Pathogens.Metabolites · 2023Article
- High-throughput retrieval of target sequences from complex clone libraries using CRISPRi.Nature biotechnology · 2023Article
- Modern Trends in Natural Antibiotic Discovery.Life (Basel, Switzerland) · 2023Review
- Bacterial natural product discovery by heterologous expression.Journal of industrial microbiology & biotechnology · 2023Review
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Authors and funding
5 authors.
Funding
Abstract
The growing threat of antibiotic resistance necessitates the discovery of antibiotics that are active against resistant pathogens. Calcium-dependent antibiotics are a small family of structurally diverse acidic lipopeptides assembled by nonribosomal peptide synthetases (NRPSs) that are known to display various modes of action against antibiotic-resistant pathogens. Here we use NRPS adenylation (AD) domain sequencing to guide the identification, recovery, and cloning of the cde biosynthetic gene cluster from a soil metagenome. Heterologous expression of the cde biosynthetic gene cluster led to the production of cadasides A (1) and B (2), a subfamily of acidic lipopeptides that is distinct from previously characterized calcium-dependent antibiotics in terms of both overall structure and acidic residue rich peptide core. The cadasides inhibit the growth of multidrug-resistant Gram-positive pathogens by disrupting cell wall biosynthesis in the presence of high concentrations of calcium. Interestingly, sequencing of AD domains from diverse soils revealed that sequences predicted to arise from cadaside-like gene clusters are predominantly found in soils containing high levels of calcium carbonate.
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