ArticleMolecular ecology resources2019
Bioaerosol biomonitoring: Sampling optimization for molecular microbial ecology.
Article in Molecular ecology resources, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed.
- Centrifugal microfluidics for rapid target analyte quantification in airborne bioaerosols.Lab on a chip · 2026Article
- Spatial and temporal patterns of public transit aerobiomes.Microbiome · 2026Article
- From air to insight: the evolution of airborne DNA sequencing technologies.Microbiology (Reading, England) · 2025Review
- Efficiency of the Coriolis µ Air Sampling Device for Fungal Contamination Analysis of Indoor Air: A Case Study.Pathogens (Basel, Switzerland) · 2025Article
- SOLARIS project: a portable 3D-printed bioaerosol sampler for environmental bacterial collection.Royal Society open science · 2025Article
- Microbial exposure during recycling of domestic waste: a cross-sectional study of composition and associations with inflammatory markers.Occupational and environmental medicine · 2024Article
- Antibiotic Resistance as a Functional Characteristic of Urban Dust Particles' Microbial Communities.Biology · 2024Article
- Impact of Anthropogenic Factors on the Diversity of Microbial Communities of PM10 Air and PM100 ofBiology · 2024Article
- Public health implications of antibiotic resistance in sewage water: an epidemiological perspective.Bioresources and bioprocessing · 2024Article
- Air quality and mental illness: role of bioaerosols, causal mechanisms and research priorities.BJPsych open · 2024Review
- Mycological Methods for Routine Air Sampling and Interpretation of Results in Operating Theaters.Diagnostics (Basel, Switzerland) · 2024Review
- The atmosphere: a transport medium or an active microbial ecosystem?The ISME journal · 2024Review
- An Optimized Active Sampling Procedure for Aerobiological DNA Studies.Sensors (Basel, Switzerland) · 2023Article
- AirDNA sampler: An efficient and simple device enabling high-yield, high-quality airborne environment DNA for metagenomic applications.PloS one · 2023Article
- Airborne transmission of biological agents within the indoor built environment: a multidisciplinary review.Air quality, atmosphere, & health · 2023Review
- Performance Evaluation and Validation of Air Samplers To Detect Aerosolized Coxiella burnetii.Microbiology spectrum · 2022Article
- Application of Microfluidic Chips in the Detection of Airborne Microorganisms.Micromachines · 2022Review
- Detection and characterization of bioaerosol emissions from wastewater treatment plants: Challenges and opportunities.Frontiers in microbiology · 2022Review
- Environmental Exposures and Lung Aging: Molecular Mechanisms and Implications for Improving Respiratory Health.Current environmental health reports · 2021Review
- Challenges and Perspectives for Biosensing of Bioaerosol Containing Pathogenic Microorganisms.Micromachines · 2021Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Bioaerosols (or biogenic aerosols) have largely been overlooked by molecular ecologists. However, this is rapidly changing as bioaerosols play key roles in public health, environmental chemistry and the dispersal ecology of microbes. Due to the low environmental concentrations of bioaerosols, collecting sufficient biomass for molecular methods is challenging. Currently, no standardized methods for bioaerosol collection for molecular ecology research exist. Each study requires a process of optimization, which greatly slows the advance of bioaerosol science. Here, we evaluated air filtration and liquid impingement for bioaerosol sampling across a range of environmental conditions. We also investigated the effect of sampling matrices, sample concentration strategies and sampling duration on DNA yield. Air filtration using polycarbonate filters gave the highest recovery, but due to the faster sampling rates possible with impingement, we recommend this method for fine -scale temporal/spatial ecological studies. To prevent bias for the recovery of Gram-positive bacteria, we found that the matrix for impingement should be phosphate-buffered saline. The optimal method for bioaerosol concentration from the liquid matrix was centrifugation. However, we also present a method using syringe filters for rapid in-field recovery of bioaerosols from impingement samples, without compromising microbial diversity for high -throughput sequencing approaches. Finally, we provide a resource that enables molecular ecologists to select the most appropriate sampling strategy for their specific research question.
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Registered trials
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