Evidence map›Paper›PMID 30735543›Full record

ArticlePloS one2019

Cloning and functional analysis of the promoter of a stress-inducible gene (Zmap) in maize.

Bo Jin, Zunlai Sheng, Ishfaq Muhammad, Jianqing Chen, Hongliang Yang

Open access · goldAbstract read
In one paragraph

Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
3.5field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 19 citations in OpenAlex.

  1. Molecular Dissection of theInternational journal of molecular sciences · 2026
    Article
  2. Article
  3. The melonPlant direct · 2024
    Article
  4. Review
  5. Article
  6. Article
  7. Stress-ResponsiveFrontiers in plant science · 2021
    Article
  8. Article
  9. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Bo JinCollege of Veterinary Medicine, Northeast Agricultural University, Harbin, P. R. China.
Zunlai ShengCollege of Veterinary Medicine, Northeast Agricultural University, Harbin, P. R. China.
Ishfaq MuhammadCollege of Veterinary Medicine, Northeast Agricultural University, Harbin, P. R. China.
Jianqing ChenCollege of Veterinary Medicine, Northeast Agricultural University, Harbin, P. R. China.
Hongliang YangCollege of Veterinary Medicine, Northeast Agricultural University, Harbin, P. R. China.ORCID 0000-0002-5718-9145
Northeast Agricultural University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The anionic peroxidases play an important role in a variety of plant physiological processes. We characterized and isolated the Zmap promoter (PZmap) at the 5' flanking region in order to better understand the regulatory mechanisms of Zmap gene expression. A series of PZmap deletion derivatives, termed a1 -a6, at positions -1694, -1394, -1138, -784, -527 and -221 from the translation start site were blended to the β-glucuronidase reporter gene. Agrobacterium-mediated transformation method was used to study each deletion construct in tobaccos. Sequence analysis showed that several cis-acting elements (MYB binding site, Box-II, a TGACG-element, a CGTCA-element and a low temperature responsive element) were located within the promoter. Deletion analysis suggested the sequence between -1,694 and -1394bp may contain cis-elements associated with GUS up regulation. The MYB binding site (-757) might act as a negative drought-responsive element. There might be repressor elements located in the region (-1,694 to -1394bp) to repress Zmap expression under 4°C. The characterized promoter would be an ideal candidate for genetic engineering for improving the resistance of maize to different stressors.

Indexed as

Gene Expression Regulation, PlantPromoter Regions, GeneticAgrobacterium tumefaciensCloning, MolecularCold TemperatureDroughtsGenes, ReporterGlucuronidaseNicotianaPlant LeavesPlant ProteinsPlants, Genetically ModifiedPlasmidsStress, PhysiologicalTransformation, GeneticZea maysGlucuronidasePlant Proteins

Identifiers

PMID30735543
PMCPMC6368321
OpenAlexW2914317754

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.