ReviewCurrent protein & peptide science2019
Biotechnological Applications of Protein Splicing.
Review in Current protein & peptide science, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed, 46 citations in OpenAlex.
- A protein chaperone can stabilize the intein-containing precursor to indirectly promote protein splicing.Protein science : a publication of the Protein Society · 2026Article
- A Unique THN Motif Is Critical for Enabling Efficient C-Terminal Traceless Cleavage.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Monitoring Protein Stability In Vivo Using an Intein-Based Biosensor.Bio-protocol · 2025Article
- Controllable intein splicing and N-terminal cleavage at mesophilic temperatures.Frontiers in bioengineering and biotechnology · 2025Article
- Anti-windup strategies for biomolecular control systems facilitated by model reduction theory for sequestration networks.Science advances · 2024Article
- Synthetic gene circuit evolution: Insights and opportunities at the mid-scale.Cell chemical biology · 2024Review
- Article
- An intein-based biosensor to measure protein stability in vivo.Protein science : a publication of the Protein Society · 2024Article
- Backbone Cyclization of Flavin Mononucleotide-Based Fluorescent Protein Increases Fluorescence and Stability.Journal of microbiology and biotechnology · 2023Article
- Massive intein content inProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Efficient splicing of the CPE intein derived from directed evolution of theActa biochimica et biophysica Sinica · 2023Article
- A cybergenetic framework for engineering intein-mediated integral feedback control systems.Nature communications · 2023Article
- Strategies for Conditional Regulation of Proteins.JACS Au · 2023Review
- Inteins-mechanism of protein splicing, emerging regulatory roles, and applications in protein engineering.Frontiers in microbiology · 2023Review
- Chromatographic single-step purification of tagless proteins using gp41-1 split inteins.Frontiers in bioengineering and biotechnology · 2023Article
- An artificially split class 3 intein.microPublication biology · 2023Article
- Editorial: Genetically mobile elements repurposed by nature and biotechnologists.Frontiers in molecular biosciences · 2022Article
- Kinetics study of the natural split Npu DnaE intein in the generation of bispecific IgG antibodies.Applied microbiology and biotechnology · 2022Article
- Protein Splicing of Inteins: A Powerful Tool in Synthetic Biology.Frontiers in bioengineering and biotechnology · 2022Review
- Reactive Chlorine Species Reversibly Inhibit DnaB Protein Splicing in Mycobacteria.Microbiology spectrum · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Protein splicing domains, also called inteins, have become a powerful biotechnological tool for applications involving molecular biology and protein engineering. Early applications of inteins focused on self-cleaving affinity tags, generation of recombinant polypeptide α-thioesters for the production of semisynthetic proteins and backbone cyclized polypeptides. The discovery of naturallyoccurring split-inteins has allowed the development of novel approaches for the selective modification of proteins both in vitro and in vivo. This review gives a general introduction to protein splicing with a focus on their role in expanding the applications of intein-based technologies in protein engineering and chemical biology.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.