Evidence map›Paper›PMID 30733838›Full record

ArticleDisease markers2019

DIAPH1 Is Upregulated and Inhibits Cell Apoptosis through ATR/p53/Caspase-3 Signaling Pathway in Laryngeal Squamous Cell Carcinoma.

Jiechao Yang, Liang Zhou, Yanping Zhang, Juan Zheng, Jian Zhou, Zheqiang Wei, Jiaping Zou

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Disease markers, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
2.5field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 34 citations in OpenAlex.

  1. DIAPH2 gene polymorphisms and laryngeal cancer risk in men.Journal of cancer research and clinical oncology · 2024
    Article
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  7. Susceptibility of cytoskeletal-associated proteins for tumor progression.Cellular and molecular life sciences : CMLS · 2021
    Review
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  10. Review
  11. DIAPH2, PTPRD and HIC1 Gene Polymorphisms and Laryngeal Cancer Risk.International journal of environmental research and public health · 2021
    Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Laryngeal Squamous Cell Carcinoma: Potential Molecular Mechanism and Prognostic Signature Based on Immune-Related Genes.Medical science monitor : international medical journal of experimental and clinical research · 2020
    Article
  17. Protein diaphanous homolog 1 (Diaph1) promotes myofibroblastic activation of hepatic stellate cells by regulating Rab5a activity and TGFβ receptor endocytosis.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2020
    Article
  18. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Jiechao YangDepartment of Otorhinolaryngology-Head and Neck Surgery, The Third People's Hospital, Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu, China.ORCID 0000-0002-5426-383X
Liang ZhouDepartment of Otorhinolaryngology-Head and Neck Surgery, Eye, Ear, Nose and Throat Hospital, Fudan University, Shanghai, China.ORCID 0000-0002-8588-2959
Yanping ZhangLaboratory of Eye, Ear, Nose and Throat Hospital, Fudan University, Shanghai, China.
Juan ZhengDepartment of Otorhinolaryngology-Head and Neck Surgery, Eye, Ear, Nose and Throat Hospital, Fudan University, Shanghai, China.
Jian ZhouDepartment of Otorhinolaryngology-Head and Neck Surgery, Eye, Ear, Nose and Throat Hospital, Fudan University, Shanghai, China.
Zheqiang WeiDepartment of Pathology, The Third People's Hospital, Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu, China.
Jiaping ZouDepartment of Otorhinolaryngology-Head and Neck Surgery, The Third People's Hospital, Affiliated Hospital of Jiangnan University, Wuxi, Jiangsu, China.ORCID 0000-0001-9864-6464
Fudan University · CNWuxi Fourth People's Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cancer bioinformatics has been used to screen possible key cancer genes and pathways. Here, through bioinformatics analysis, we found that high expression of diaphanous related formin 1 (DIAPH1) was associated with poor overall survival in head and neck squamous cell carcinoma and laryngeal squamous cell carcinoma (LSCC). The effect of DIAPH1 in LSCC has not been previously investigated. Therefore, we evaluated the expression, function, and molecular mechanisms of DIAPH1 in LSCC. Immunohistochemistry and western blot analysis confirmed the significant upregulation of DIAPH1 in LSCC. We used DIAPH1 RNA interference to construct two DIAPH1-knockdown LSCC cell lines, AMC-HN-8 and FD-LSC-1, and validated the knockdown efficiency. Flow cytometry data showed that DIAPH1 inhibited apoptosis. Further, western blot analysis revealed that DIAPH1 knockdown increased the protein levels of ATR, p-p53, Bax, and cleaved caspase-3, -8, and -9. Thus, DIAPH1 is upregulated in LSCC and may act as an oncogene by inhibiting apoptosis through the ATR/p53/caspase-3 pathway in LSCC cells.

Indexed as

ApoptosisGene Expression Regulation, NeoplasticSignal TransductionAdaptor Proteins, Signal TransducingAtaxia Telangiectasia Mutated ProteinsCarcinoma, Squamous CellCaspase 3Cell Line, TumorForminsHumansLaryngeal NeoplasmsMiddle AgedTumor Suppressor Protein p53Up-RegulationAdaptor Proteins, Signal TransducingAtaxia Telangiectasia Mutated ProteinsATR protein, humanCaspase 3DIAPH1 protein, humanForminsTP53 protein, humanTumor Suppressor Protein p53

Identifiers

PMID30733838
PMCPMC6348834
OpenAlexW2911013141

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.