ArticleScientific reports2019
Simultaneous targeting of linked loci in mouse embryos using base editing.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Mechanistic machine learning for prediction of prime editing outcomes.Nature biotechnology · 2026Article
- Mechanistic machine learning enables interpretable and generalizable prediction of prime editing outcomes.bioRxiv : the preprint server for biology · 2026Article
- Genome editing: An insight into disease resistance, production efficiency, and biomedical applications in livestock.Functional & integrative genomics · 2024Review
- How CRISPR Is Revolutionizing the Generation of New Models for Cancer Research.Cold Spring Harbor perspectives in medicine · 2024Review
- Cell-specific and shared regulatory elements control a multigene locus active in mammary and salivary glands.Nature communications · 2023Article
- Base editing and prime editing in laboratory animals.Laboratory animals · 2022Article
- Prime editing in mice reveals the essentiality of a single base in driving tissue-specific gene expression.Genome biology · 2021Article
- Development of CRISPR technology for precise single-base genome editing: a brief review.BMB reports · 2021Review
- Precision genome editing using cytosine and adenine base editors in mammalian cells.Nature protocols · 2021Article
- Advances in Genome Editing and Application to the Generation of Genetically Modified Rat Models.Frontiers in genetics · 2021Review
- Genome editing with CRISPR-Cas nucleases, base editors, transposases and prime editors.Nature biotechnology · 2020Review
- Disease modeling and stem cell immunoengineering in regenerative medicine using CRISPR/Cas9 systems.Computational and structural biotechnology journal · 2020Review
- Single-nucleotide editing: From principle, optimization to application.Human mutation · 2019Review
- CRISPR links to long noncoding RNA function in mice: A practical approach.Vascular pharmacology · 2019Review
- Targeting fidelity of adenine and cytosine base editors in mouse embryos.Nature communications · 2018Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
A particular challenge in genome engineering has been the simultaneous introduction of mutations into linked (located on the same chromosome) loci. Although CRISPR/Cas9 has been widely used to mutate individual sites, its application in simultaneously targeting of linked loci is limited as multiple nearby double-stranded DNA breaks created by Cas9 routinely result in the deletion of sequences between the cleavage sites. Base editing is a newer form of genome editing that directly converts C∙G-to-T∙A, or A∙T-to-G∙C, base pairs without introducing double-stranded breaks, thus opening the possibility to generate linked mutations without disrupting the entire locus. Through the co-injection of two base editors and two sgRNAs into mouse zygotes, we introduced C∙G-to-T∙A transitions into two cytokine-sensing transcription factor binding sites separated by 9 kb. We determined that one enhancer activates the two flanking genes in mammary tissue during pregnancy and lactation. The ability to introduce linked mutations simultaneously in one step into the mammalian germline has implications for a wide range of applications, including the functional analysis of linked cis-elements creating disease models and correcting pathogenic mutations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.