ArticleScientific reports2019
Transcriptome-based identification and validation of reference genes for plant-bacteria interaction studies using Nicotiana benthamiana.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- ABI5-binding proteins are substrates of key components in the ABA core signaling pathway affecting seeds.Plant physiology · 2026Article
- Unveiling the dual role ofPlant signaling & behavior · 2025Article
- Transcriptome-Based Identification of Reference Genes for Expression Analysis in Cassava UnderPlants (Basel, Switzerland) · 2025Article
- RepA Protein of Citrus Chlorotic Dwarf-Associated Virus Impairs Perinuclear Chloroplast Clustering Induced by Lemon Chloroplast Malate Dehydrogenase.Molecular plant pathology · 2025Article
- Recovery of RNA-dependent RNA polymerase 6 gene-knockout phenotypes in Nicotiana benthamiana via in vivo generation of inverted repeat construct of the trans-acting short interference RNA3 sequence.The Plant journal : for cell and molecular biology · 2025Article
- Transcriptome-based identification and validation of reference genes for corm growth stages, different tissues, and drought stress in Taro (Colocasia esculenta).BMC plant biology · 2024Article
- 'Candidatus Phytoplasma mali' SAP11-Like protein modulates expression of genes involved in energy production, photosynthesis, and defense in Nicotiana occidentalis leaves.BMC plant biology · 2024Article
- Article
- Phosphatidylinositol-phospholipase C3 negatively regulates the hypersensitive response via complex signaling with MAP kinase, phytohormones, and reactive oxygen species in Nicotiana benthamiana.Journal of experimental botany · 2023Article
- Selection and Validation of Reference Genes for RT-qPCR Analysis of Gene Expression inPlants (Basel, Switzerland) · 2023Article
- An atypical NLR protein modulates the NRC immune receptor network in Nicotiana benthamiana.PLoS genetics · 2023Article
- Article
- Analysis of controlling genes for tiller growth of Psathyrostachys juncea based on transcriptome sequencing technology.BMC plant biology · 2022Article
- CRISPR/Cas9-mediated knockout of the DCL2 and DCL4 genes in Nicotiana benthamiana and its productivity of recombinant proteins.Plant cell reports · 2022Article
- Selection of potential reference genes for RT-qPCR in the plant pathogenic fungusFrontiers in microbiology · 2022Article
- Identification of stably expressed reference genes for expression studies inFrontiers in plant science · 2022Article
- Public transcriptome database-based selection and validation of reliable reference genes for breast cancer research.Biomedical engineering online · 2021Article
- Selection of a reference gene for studies on lipid-related aquatic adaptations of toothed whales (Ecology and evolution · 2021Article
- Identification ofInternational journal of molecular sciences · 2021Article
- Siberian Wildrye (Plants (Basel, Switzerland) · 2021Article
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6 authors.
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No grant is acknowledged in the PubMed record.
Abstract
RT-qPCR is a widely used technique for the analysis of gene expression. Accurate estimation of transcript abundance relies strongly on a normalization that requires the use of reference genes that are stably expressed in the conditions analyzed. Initially, they were adopted from those used in Northern blot experiments, but an increasing number of publications highlight the need to find and validate alternative reference genes for the particular system under study. The development of high-throughput sequencing techniques has facilitated the identification of such stably expressed genes. Nicotiana benthamiana has been extensively used as a model in the plant research field. In spite of this, there is scarce information regarding suitable RT-qPCR reference genes for this species. Employing RNA-seq data previously generated from tomato plants, combined with newly generated data from N. benthamiana leaves infiltrated with Pseudomonas fluorescens, we identified and tested a set of 9 candidate reference genes. Using three different algorithms, we found that NbUbe35, NbNQO and NbErpA exhibit less variable gene expression in our pathosystem than previously used genes. Furthermore, the combined use of the first two is sufficient for robust gene expression analysis. We encourage employing these novel reference genes in future RT-qPCR experiments involving N. benthamiana and Pseudomonas spp.
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