ArticleScientific reports2019
The major allergen Der p 2 is a cholesterol binding protein.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 39 citations in OpenAlex.
- Evaluation of the Biological Standardization of Native Der p 1, Der p 2 and Der p 23 Proteins Isolated from Natural Allergen Source.International journal of molecular sciences · 2026Article
- Transient Expression and Immunogenicity Assessment of theVaccines · 2026Article
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- The adeno associated viral vectored Dp12S vaccine effective alleviation of asthma symptoms in mice.NPJ vaccines · 2025Article
- Article
- Prick test accuracy for Der p components in pediatric rhinitis.European journal of pediatrics · 2025Article
- Characterization of a major house dust mite allergen Der p 22 among Malaysian adult population.The World Allergy Organization journal · 2025Article
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- Article
- Natural killer T cells in allergic asthma: implications for the development of novel immunotherapeutical strategies.Frontiers in immunology · 2024Review
- Insight Into the Dynamics of the Ixodes ricinus Nymphal Midgut Proteome.Molecular & cellular proteomics : MCP · 2023Article
- Role of Small Molecule Ligands in IgE-Mediated Allergy.Current allergy and asthma reports · 2023Review
- Co-Activation of Human Whole Blood Cells with Lipopolysaccharides and an Allergen.Life (Basel, Switzerland) · 2023Article
- Determination of immunogenic epitopes in major house dust mite allergen, Der p 2, via nanoallergens.Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology · 2022Article
- Integrated OMICs Approach for the Group 1 Protease Mite-Allergen of House Dust MiteInternational journal of molecular sciences · 2022Article
- Molecular Profile Sensitization to House Dust Mites as an Important Aspect for Predicting the Efficiency of Allergen Immunotherapy.Frontiers in immunology · 2022Article
- Allergens and their associated small molecule ligands-their dual role in sensitization.Allergy · 2021Review
- Transcriptomic analysis of the poultry red mite, Dermanyssus gallinae, across all stages of the lifecycle.BMC genomics · 2021Article
- Removal and Replacement of Endogenous Ligands from Lipid-Bound Proteins and Allergens.Journal of visualized experiments : JoVE · 2021Article
- Cat-NPC2, a Newly Identified Allergen, With High Cross-Reactivity to Can f 7.Allergy, asthma & immunology research · 2021Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Der p 2 is a major dust mite allergen and >80% of mite allergic individuals have specific IgE to this allergen. Although it is well characterized in terms of allergenicity, there is still some ambiguity in terms of its biological function. Three-dimensional structural analysis of Der p 2 and its close homologues indicate the presence of a hydrophobic cavity which can potentially bind to lipid molecules. In this study, we aimed to identify the potential ligand of Der p 2. Using a liposome pulldown assay, we show that recombinant Der p 2 binds to liposomes prepared with exogenous cholesterol in a dose dependent fashion. Next, an ELISA based assay using immobilized lipids was used to study binding specificities of other lipid molecules. Cholesterol was the preferred ligand of Der p 2 among 11 different lipids tested. Two homologues of Der p 2, Der f 2 and Der f 22 also bound to cholesterol. Further, using liquid chromatography-mass spectrometry (LC-MS), we confirmed that cholesterol is the natural ligand of Der p 2. Three amino acid residues of Der p 2, V104, V106 and V110 are possible cholesterol binding sites, as alanine mutations of these residues showed a significant decrease in binding (p < 0.05) compared to wild-type Der p 2. These results provide the first direct experimental evidence that Der p 2 binds to cholesterol.
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