Evidence map›Paper›PMID 30732651›Full record

ArticleMicrobial cell factories2019

Establishment of BmoR-based biosensor to screen isobutanol overproducer.

Huan Yu, Ning Wang, Wenbo Huo, Yuhong Zhang, Wei Zhang, Yu Yang, Zhenya Chen, Yi-Xin Huo

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbial cell factories, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.

0numbers the graph read from it
0cells of the map it votes in
26citing papers in PubMed
5.8field-weighted citation impact, top 3% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

26 citing papers in PubMed, 88 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Article
  6. Review
  7. Review
  8. Article
  9. ACS omega · 2023
    Review
  10. Advances in biosynthesis of higher alcohols in Escherichia coli.World journal of microbiology & biotechnology · 2023
    Review
  11. Review
  12. Review
  13. Toward bioproduction of oxo chemicals from CBiotechnology for biofuels and bioproducts · 2022
    Review
  14. Article
  15. Review
  16. Review
  17. An Artificial Pathway forFrontiers in microbiology · 2022
    Article
  18. Elucidation of Sequence-Function Relationships for an Improved BiobutanolFrontiers in bioengineering and biotechnology · 2022
    Article
  19. Review
  20. Recent trends in biocatalysis.Chemical Society reviews · 2021
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Huan YuKey Laboratory of Molecular Medicine and Biotherapy, School of Life Science, Beijing Institute of Technology, No. 5 South Zhongguancun Street, Beijing, 100081, China.
Ning WangKey Laboratory of Molecular Medicine and Biotherapy, School of Life Science, Beijing Institute of Technology, No. 5 South Zhongguancun Street, Beijing, 100081, China.
Wenbo HuoKey Laboratory of Molecular Medicine and Biotherapy, School of Life Science, Beijing Institute of Technology, No. 5 South Zhongguancun Street, Beijing, 100081, China.
Yuhong ZhangBiotechnology Research Institute of Chinese Academy of Agricultural Sciences, No. 12 South Zhongguancun Street, Beijing, 100081, China.
Wei ZhangBiotechnology Research Institute of Chinese Academy of Agricultural Sciences, No. 12 South Zhongguancun Street, Beijing, 100081, China.
Yu YangKey Laboratory of Molecular Medicine and Biotherapy, School of Life Science, Beijing Institute of Technology, No. 5 South Zhongguancun Street, Beijing, 100081, China.
Zhenya ChenKey Laboratory of Molecular Medicine and Biotherapy, School of Life Science, Beijing Institute of Technology, No. 5 South Zhongguancun Street, Beijing, 100081, China. chenzhenya@bit.edu.cn.
Yi-Xin HuoKey Laboratory of Molecular Medicine and Biotherapy, School of Life Science, Beijing Institute of Technology, No. 5 South Zhongguancun Street, Beijing, 100081, China. huoyixin@bit.edu.cn.ORCID http://orcid.org/0000-0003-4644-999X
Beijing Institute of Technology · CNChinese Academy of Agricultural Sciences · CN

Funding

China Postdoctoral Science Foundation 2018M630083National Natural Science Foundation of China 21676026
6 · The paper itself

Abstract

backgroundIsobutanol, a C4 branched-chain higher alcohol, is regarded as an attractive next-generation transport fuel. Metabolic engineering for efficient isobutanol production has been achieved in many studies. BmoR, an alcohol-regulated transcription factor, mediates a σ

resultsFirstly, we constructed and verified a GFP-based BmoR-P

conclusionsThis work screened out isobutanol overproduction strains from a mutagenesis library by using a screening system which depended on the combination of BmoR-based biosensor and isobutanol biosynthetic pathway. Optimizing fermentation condition and reinforcing upstream pathway could realize the increase of isobutanol production from the overproducer. Lastly, fed-batch fermentation of the mutant enhanced the isobutanol production to 56.5 ± 1.8 g/L.

Indexed as

Biosensing TechniquesBiosynthetic PathwaysButanolsFermentationIndustrial MicrobiologyMetabolic EngineeringMutagenesisMutationThaueraButanolsisobutyl alcoholBiosensorBmoRIsobutanolMutagenesisScreening

Identifiers

PMID30732651
PMCPMC6366067
OpenAlexW2914477646

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.