ArticleMicrobial cell factories2019
Direct pathway cloning of the sodorifen biosynthetic gene cluster and recombinant generation of its product in E. coli.
Article in Microbial cell factories, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 54 citations in OpenAlex.
- Heterologous expression of NRPS and PKS pathways in Escherichia coli: from bottlenecks to biosynthetic platforms.Essays in biochemistry · 2026Review
- A guide to Direct Pathway Cloning (DiPaC) for natural product discovery.Essays in biochemistry · 2026Review
- The Maculalactone Biosynthetic Gene Cluster, a Cryptic Furanolide Pathway Revealed inACS synthetic biology · 2026Article
- Discovery and Biosynthesis of Farnesyl Pyrophosphate-Derived Noncanonical CJournal of the American Chemical Society · 2026Article
- Homoterpene Biosynthesis in Fungi.Angewandte Chemie (International ed. in English) · 2025Article
- Phosphorylation of peptides by a kinase domain in cyanobactin pathways.Nature communications · 2025Article
- Integrative Multi-Omics Identify Key Secondary Metabolites Linked to Acid Tolerance inMicroorganisms · 2025Article
- Heterologous Expression and Optimization of Fermentation Conditions for Recombinant Ikarugamycin Production.Biotechnology and bioengineering · 2025Article
- Heterologous Expression of a Cryptic BGC fromACS synthetic biology · 2025Article
- Bacillamide D produced by Bacillus cereus from the mouse intestinal bacterial collection (miBC) is a potent cytotoxin in vitro.Communications biology · 2024Article
- The microbial biosynthesis of noncanonical terpenoids.Applied microbiology and biotechnology · 2024Review
- Widespread biosynthesis of 16-carbon terpenoids in bacteria.Nature chemical biology · 2023Article
- Annexin A7 and Its Related Protein Suppressor of Death Domains Regulates Migration and Proliferation of Hca-P Cells.Cell journal · 2023Article
- Review
- Fragmentation and [4 + 3] cycloaddition in sodorifen biosynthesis.Nature chemistry · 2023Article
- Discovery and Heterologous Expression of Microginins fromACS synthetic biology · 2022Article
- Expanding the terpene biosynthetic code with non-canonical 16 carbon atom building blocks.Nature communications · 2022Article
- Biosynthesis of cyanobacterin, a paradigm for furanolide core structure assembly.Nature chemical biology · 2022Article
- Biotechnological applications of S-adenosyl-methionine-dependent methyltransferases for natural products biosynthesis and diversification.Bioresources and bioprocessing · 2021Review
- Bacterial terpenome.Natural product reports · 2021Review
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
backgroundSerratia plymuthica WS3236 was selected for whole genome sequencing based on preliminary genetic and chemical screening indicating the presence of multiple natural product pathways. This led to the identification of a putative sodorifen biosynthetic gene cluster (BGC). The natural product sodorifen is a volatile organic compound (VOC) with an unusual polymethylated hydrocarbon bicyclic structure (C
resultsSequencing and mining of the S. plymuthica WS3236 genome revealed the presence of 38 BGCs according to antiSMASH analysis, including a putative sodorifen BGC. Further genome mining for sodorifen and sodorifen-like BGCs throughout bacteria was performed using SodC and SodD as queries and identified a total of 28 sod-like gene clusters. Using direct pathway cloning (DiPaC) we intercepted the 4.6 kb candidate sodorifen BGC from S. plymuthica WS3236 (sodA-D) and transformed it into Escherichia coli BL21. Heterologous expression under the control of the tetracycline inducible Ptet
conclusionsGenome mining of all available genomes within the NCBI and JGI IMG databases led to the identification of a wealth of sod-like pathways which may be responsible for producing a range of structurally unknown sodorifen analogs. Introduction of the S. plymuthica WS3236 sodorifen BGC into the fast-growing heterologous expression host E. coli with a very low VOC background led to a significant increase in both sodorifen product yield and purity compared to the native producer. By providing a reliable, high-level production system, this study sets the stage for future investigations of the biological role and function of sodorifen and for functionally unlocking the bioinformatically identified putative sod-like pathways.
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