Evidence map›Paper›PMID 30729921›Full record

ArticleParasite (Paris, France)2019

Molecular identification of Wolbachia and Sodalis glossinidius in the midgut of Glossina fuscipes quanzensis from the Democratic Republic of Congo.

Gustave Simo, Sartrien Tagueu Kanté, Joule Madinga, Ginette Kame, Oumarou Farikou, Gillon Ilombe, Anne Geiger, Pascal Lutumba, Flobert Njiokou

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Parasite (Paris, France), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Gustave SimoMolecular Parasitology and Entomology Unit, Department of Biochemistry, Faculty of Science, University of Dschang, PO Box 67, Dschang, Cameroon.
Sartrien Tagueu KantéMolecular Parasitology and Entomology Unit, Department of Biochemistry, Faculty of Science, University of Dschang, PO Box 67, Dschang, Cameroon.
Joule MadingaInstitute of Health and Society, Université Catholique de Louvain, Clos Chapelle-aux-Champs 30, 1200 Woluwe-Saint-Lambert, Brussels, Belgium - Department of Biomedical Sciences, Institute of Tropical Medicine, Nationalestraat 155, 2000, Antwerp, Belgium.
Ginette KameDepartment of Animal Biology and Physiology, Faculty of Science, University of Yaoundé I, PO Box 812 Yaoundé, Cameroon.
Oumarou FarikouMission Spéciale d'Eradication des Glossines, Division Régionale Tsé-Tsé Adamaoua, PO Box 263 Ngaoundéré, Cameroon.
Gillon IlombeInstitut national de recherche biomédicale Kinshasa, Avenue de la démocratie N°5345, Gombe, Kinshasa, Democratic Republic of Congo.
Anne GeigerUMR 177, IRD-CIRAD, CIRAD TA A-17/G, Campus International de Baillarguet, Montpellier Cedex 5, France - Center for Research on Filariasis and other Tropical Diseases (CRFILMT), PO Box 5797 Yaoundé, Cameroon - University of Yaoundé I, Faculty of Science, PO Box 812, Yaoundé, Cameroon.
Pascal LutumbaInstitut national de recherche biomédicale Kinshasa, Avenue de la démocratie N°5345, Gombe, Kinshasa, Democratic Republic of Congo - Department of Tropical Medicine, University of Kinshasa, B.P. 127 Kinshasa XI, Democratic Republic of Congo.
Flobert NjiokouDepartment of Animal Biology and Physiology, Faculty of Science, University of Yaoundé I, PO Box 812 Yaoundé, Cameroon.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

During the last 30 years, investigations on the microbiome of different tsetse species have generated substantial data on the bacterial flora of these cyclical vectors of African trypanosomes, with the overarching goal of improving the control of trypanosomiases. It is in this context that the presence of Wolbachia and Sodalis glossinidius was studied in wild populations of Glossina fuscipes quanzensis from the Democratic Republic of Congo. Tsetse flies were captured with pyramidal traps. Of the 700 Glossina f. quanzensis captured, 360 were dissected and their midguts collected and analyzed. Sodalis glossinidius and Wolbachia were identified by PCR. The Wolbachia-positive samples were genetically characterized with five molecular markers. PCR revealed 84.78% and 15.55% midguts infected by Wolbachia and S. glossinidius, respectively. The infection rates varied according to capture sites. Of the five molecular markers used to characterize Wolbachia, only the fructose bis-phosphate aldolase gene was amplified for about 60% of midguts previously found with Wolbachia infections. The sequencing results confirmed the presence of Wolbachia and revealed the presence of S. glossinidius in the midgut of Glossina f. quanzensis. A low level of midguts were naturally co-infected by both bacteria. The data generated in this study open a framework for investigations aimed at understanding the contribution of these symbiotic microorganisms to the vectorial competence of Glossina fuscipes quanzensis.

Indexed as

AnimalsCoinfectionDemocratic Republic of the CongoDigestive SystemDNA, BacterialEnterobacteriaceaeFructose-Bisphosphate AldolaseHigh-Throughput Nucleotide SequencingInsect VectorsPolymerase Chain ReactionSymbiosisTsetse FliesWolbachiaDNA, BacterialFructose-Bisphosphate Aldolase

Identifiers

PMID30729921
PMCPMC6366345

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.