ArticleMicrobiologyOpen2019
Characterization of a phospholipid-regulated β-galactosidase from Akkermansia muciniphila involved in mucin degradation.
Article in MicrobiologyOpen, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it, 36 citations in OpenAlex.
- The role ofFrontiers in medicine · 2025Pooled it
- Functional Proteins of Akkermansia Muciniphila: Impacts on Host Health and Metabolism.Probiotics and antimicrobial proteins · 2026Review
- Insights into the Functions, Characteristics, and Mechanisms of Disease-Related Proteins fromMicroorganisms · 2026Review
- Proteomic study ofFrontiers in microbiology · 2025Article
- Mechanism of 2'-fucosyllactose degradation by human-associatedJournal of bacteriology · 2024Article
- A genetic system for Akkermansia muciniphila reveals a role for mucin foraging in gut colonization and host sterol biosynthesis gene expression.Nature microbiology · 2023Article
- The Effects of a High-Fat Diet on Inflammatory Bowel Disease.Biomolecules · 2023Review
- O-Mucin-degrading carbohydrate-active enzymes and their possible implication in inflammatory bowel diseases.Essays in biochemistry · 2023Review
- Capsaicin modulates Akkermansia muciniphila abundance by enhancing MUCIN2 levels in mice fed with high-fat diets.Food & nutrition research · 2023Article
- How microbial glycosyl hydrolase activity in the gut mucosa initiates microbial cross-feeding.Glycobiology · 2022Review
- Identification of α-L-fucosidase (ALFuc) of Blastocystis sp. subtypes ST1, ST2 and ST3.Revista do Instituto de Medicina Tropical de Sao Paulo · 2022Article
- Review
- Leonurine protects against ulcerative colitis by alleviating inflammation and modulating intestinal microflora in mouse models.Experimental and therapeutic medicine · 2021Article
- Transcriptomics and metabolomics reveal the adaption of Akkermansia muciniphila to high mucin by regulating energy homeostasis.Scientific reports · 2021Article
- Proteomics and Metaproteomics Add Functional, Taxonomic and Biomass Dimensions to Modeling the Ecosystem at the Mucosal-luminal Interface.Molecular & cellular proteomics : MCP · 2020Review
- Akkermansia muciniphila uses human milk oligosaccharides to thrive in the early life conditions in vitro.Scientific reports · 2020Article
- Gut bacteria affect the tumoral immune milieu: distorting the efficacy of immunotherapy or not？.Gut microbes · 2020Review
- Characterization of a phospholipid-regulated β-galactosidase from Akkermansia muciniphila involved in mucin degradation.MicrobiologyOpen · 2019Article
- Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The gut microbe Akkermansia muciniphila is important for the human health as the occurrence of the organism is inversely correlated with different metabolic disorders. The metabolism of the organism includes the degradation of intestinal mucins. Thus, the gut health-promoting properties are not immediately obvious and mechanisms of bacteria-host interactions are mostly unclear. In this study, we characterized a novel extracellular β-galactosidase (Amuc_1686) with a preference for linkages from the type Galβ1-3GalNAc. Additionally, Amuc_1686 possesses a discoidin-like domain, which enables the interaction with anionic phospholipids. We detected a strong inhibition by phosphatidylserine, phosphatidylglycerol, phosphatidic acid, and lysophosphatidic acid while phosphatidylcholine and phosphatidylethanolamine had no influence. Amuc_1686 is the first example of a prokaryotic hydrolase that is strongly inhibited by certain phospholipids. These inhibiting phospholipids have important signal functions in immune response and cell clearance processes. Hence, Amuc_1686 might be regulated based on the health status of the large intestine and could therefore contribute to the mutualistic relationship between the microbe and the host on a molecular level. In this sense, Amuc_1686 could act as an altruistic enzyme that does not attack the mucin layer of apoptotic epithelial cells to ensure tissue regeneration, for example, in areas with inflammatory damages.
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Registered trials
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