ArticleIndian journal of clinical biochemistry : IJCB2019
Methods for Isolation of High Quality and Quantity of miRNA and Single Cell Suspension for Flow-Cytometry from Breast Cancer Tissue: A Comparative Analysis.
Article in Indian journal of clinical biochemistry : IJCB, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 10 citations in OpenAlex.
- MicroRNA Significance in Cancer: An Updated Review on Diagnostic, Prognostic, and Therapeutic Perspectives.EJIFCC · 2024Review
- Combined DietaryVeterinary sciences · 2024Article
- Downregulation of miRNA-21 and cancer stem cells after chemotherapy results in better outcome in breast cancer patients.World journal of stem cells · 2022Article
- Single Cell Omics of Breast Cancer: An Update on Characterization and Diagnosis.Indian journal of clinical biochemistry : IJCB · 2019Review
- Single Cell Omics Approach: A Paradigm Shift in Diagnosis and Therapy of Cancer.Indian journal of clinical biochemistry : IJCB · 2019Article
- Stem Cell Biology: A New Hope in Regenerations and Replenishments Therapy.Indian journal of clinical biochemistry : IJCB · 2018Article
- Tissue-Resident Memory Cells: New Marked Shield to Fight Cancers.Indian journal of clinical biochemistry : IJCB · 2018Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
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Abstract
Inadequate methods may cause substantial loss not only in the quantity but also in quality of the product. This study aimed to determine the best method for making single cell suspension for isolation of RNA and flow cytometer analysis from cancer tissue. We compared two methods of tissue disruption used during RNA isolation and flow cytometer analysis. Mechanical tissue disruption method and enzymatic tissue digestion method are commonly used for making single cell suspension before RNA isolation and flow cytometer analysis. 20 resected tissue samples were dissociated into single cells by mechanical and enzymatic methods. Quality and quantity of isolated miRNA was graded by the ratio of 260/280 nm and by running gels. The results revealed that mechanical hand held tissue homogenizer showed better yield than enzymatic (719.12 ± 513.67 vs. 524.87 ± 388.18 ng/µl) and the quality 260/280 nm ratio was significantly better [2.15 ± 0.21 vs. 1.57 ± 0.23; 95% CI (0.402-0.730);
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