ArticleJournal of food science and technology2019
Development of rapid dipstick assay for food pathogens, Salmonella, by optimized parameters.
Article in Journal of food science and technology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- Metal and metal oxide nanoparticle-assisted molecular assays for the detection of Salmonella.Discover nano · 2025Review
- Sensitive Immunochromatographic Determination ofNanomaterials (Basel, Switzerland) · 2023Article
- Development of nucleic acid based lateral flow assays for SARS-CoV-2 detection.Journal of bioscience and bioengineering · 2023Article
- Design and Development of Magnetic Iron Core Gold Nanoparticle-Based Fluorescent Multiplex Assay to DetectNanomaterials (Basel, Switzerland) · 2022Article
- On-site detection of food and waterborne bacteria - current technologies, challenges, and future directions.Trends in food science & technology · 2021Article
- Sorbent-incorporated dipstick for direct assaying of proteases.Analytical and bioanalytical chemistry · 2020Article
- Electrochemical Biosensors for Detection of Foodborne Pathogens.Micromachines · 2019Review
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Salmonella is among the very important pathogens threating the human and animal health. Rapid and easy detection of these pathogens is crucial. In this context, antibody (Ab) based lateral flow assays (LFAs) which are simple immunochromatographic point of care test kits were developed by gold nanoparticles (GNPs) as labelling agent for Salmonella detection. For that purpose some critical parameters such as reagent concentrations on the capture zones, conjugate concentrations and ideal membrane type needed for LFAs for whole cell detection were tested for naked eye analysis. Therefore, prepared LFAs were applied to the live and heat inactivated cells when they were used alone or included in different bacterial mixtures. Among the test platforms, membrane 180 (M180) was found as an ideal membrane and 36 nm GNPs showed highly good labelling in the developed LFAs. Diluted conjugates and low concentrations of reagents affected the test signal negatively. Salmonella was detected in different bacterial mixtures, selectively in 4-5 min. The best recognized species by used Ab were
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