ArticleScientific reports2019
The bovine alveolar macrophage DNA methylome is resilient to infection with Mycobacterium bovis.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
6 citing papers in PubMed.
- Extensive differential DNA methylation between tuberculosis skin test positive and skin test negative cattle.BMC genomics · 2024Article
- Surveying the Epigenetic Landscape of Tuberculosis in Alveolar Macrophages.Infection and immunity · 2022Review
- Integrative genomics of the mammalian alveolar macrophage response to intracellular mycobacteria.BMC genomics · 2021Article
- DNA methylation studies in cattle.Journal of applied genetics · 2021Review
- Epigenomics and genotype-phenotype association analyses reveal conserved genetic architecture of complex traits in cattle and human.BMC biology · 2020Article
- Impacts of Epigenetic Processes on the Health and Productivity of Livestock.Frontiers in genetics · 2020Review
Corrections and comments
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Authors and funding
12 authors.
Funding
Abstract
DNA methylation is pivotal in orchestrating gene expression patterns in various mammalian biological processes. Perturbation of the bovine alveolar macrophage (bAM) transcriptome, due to Mycobacterium bovis (M. bovis) infection, has been well documented; however, the impact of this intracellular pathogen on the bAM epigenome has not been determined. Here, whole genome bisulfite sequencing (WGBS) was used to assess the effect of M. bovis infection on the bAM DNA methylome. The methylomes of bAM infected with M. bovis were compared to those of non-infected bAM 24 hours post-infection (hpi). No differences in DNA methylation (CpG or non-CpG) were observed. Analysis of DNA methylation at proximal promoter regions uncovered >250 genes harbouring intermediately methylated (IM) promoters (average methylation of 33-66%). Gene ontology analysis, focusing on genes with low, intermediate or highly methylated promoters, revealed that genes with IM promoters were enriched for immune-related GO categories; this enrichment was not observed for genes in the high or low methylation groups. Targeted analysis of genes in the IM category confirmed the WGBS observation. This study is the first in cattle examining genome-wide DNA methylation at single nucleotide resolution in an important bovine cellular host-pathogen interaction model, providing evidence for IM promoter methylation in bAM.
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