ArticleBMC genomics2019
Tracing cellular heterogeneity in pooled genetic screens via multi-level barcoding.
Article in BMC genomics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed.
- High-Content CRISPR Screening: Methods and Applications.MedComm · 2026Review
- Multiplexed perturbation enables scalable pooled screens.Nature methods · 2026Article
- Genome-Wide CRISPR Screen Identifies Genes Involved in Metastasis of Pancreatic Ductal Adenocarcinoma.Cancers · 2024Article
- Genome-wide screens identify SEL1L as an intracellular rheostat controlling collagen turnover.Nature communications · 2024Article
- Charting Single Cell Lineage Dynamics and Mutation Networks via Homing CRISPR.bioRxiv : the preprint server for biology · 2024Article
- Article
- Targeted high-throughput mutagenesis of the human spliceosome reveals its in vivo operating principles.Molecular cell · 2023Article
- Dynamic and single cell characterization of a CRISPR-interference toolset inMetabolic engineering communications · 2022Article
- Designing libraries for pooled CRISPR functional screens of long noncoding RNAs.Mammalian genome : official journal of the International Mammalian Genome Society · 2022Review
- CRISPR-Cas9 library screening approach for anti-cancer drug discovery: overview and perspectives.Theranostics · 2022Review
- What sequencing technologies can teach us about innate immunity.Immunological reviews · 2022Review
- Cannabidiol converts NF-κB into a tumor suppressor in glioblastoma with defined antioxidative properties.Neuro-oncology · 2021Article
- High-Throughput CRISPR Screening Identifies Genes Involved in Macrophage Viability and Inflammatory Pathways.Cell reports · 2020Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
backgroundWhile pooled loss- and gain-of-function CRISPR screening approaches have become increasingly popular to systematically investigate mammalian gene function, the large majority of them have thus far not investigated the influence of cellular heterogeneity on screen results. Instead most screens are analyzed by averaging the abundance of perturbed cells from a bulk population of cells.
resultsHere we developed multi-level barcoded sgRNA libraries to trace multiple clonal Cas9 cell lines exposed to the same environment. The first level of barcoding allows monitoring growth kinetics and treatment responses of multiplexed clonal cell lines under identical conditions while the second level enables in-sample replication and tracing of sub-clonal lineages of cells expressing the same sgRNA.
conclusionUsing our approach, we illustrate how heterogeneity in growth kinetics and treatment response of clonal cell lines impairs the results of pooled genetic screens.
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Registered trials
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