ArticleMemorias do Instituto Oswaldo Cruz2019
Analytical validation of real-time quantitative PCR assays for optimum diagnosis of vivax malaria.
Article in Memorias do Instituto Oswaldo Cruz, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
8 citing papers in PubMed, 14 citations in OpenAlex.
- Diagnosing P. simium zoonotic malaria infection in the Rio de Janeiro Atlantic forest, Brazil.Scientific reports · 2025Article
- Tracking Drug Resistance inInternational journal of molecular sciences · 2025Article
- Novel isothermal nucleic acid amplification method for detecting malaria parasites.Applied microbiology and biotechnology · 2024Article
- A highly sensitive and specific real-time quantitative polymerase chain reaction assay for Perkinsus marinus detection in oysters.Scientific reports · 2024Article
- AreBiomedicines · 2024Article
- Molecular test for screening malaria-infected blood donors to maximise recipient safety in Acre State, a Brazilian endemic area.Memorias do Instituto Oswaldo Cruz · 2024Article
- RelapsingMalariaWorld journal · 2024Article
- Reemergence of human malaria in Atlantic Forest of Rio Grande do Sul, Brazil.Memorias do Instituto Oswaldo Cruz · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe prompt diagnosis of plasmodial species for effective treatment prevents worsening of individual health and avoids transmission maintenance or even malaria reintroduction in areas where Plasmodium does not exist. Polymerase chain reaction (PCR) allows for the detection of parasites below the threshold of microscopic examination.
objectiveOur aim was to develop a real-time PCR test to reduce diagnostic errors and increase efficacy.
methodsThe lower limit of quantification and the linearity/analytical sensitivity to measure sensitivity or limit of detection (LoD) were determined. Intra-assay variations (repeatability) and alterations between assays, operators, and instruments (reproducibility) were also assessed to set precision.
findingsThe linearity in SYBR™ Green and TaqMan™ systems was 106 and 102 copies and analytical sensitivity 1.13 and 1.17 copies/μL, respectively. Real-time PCR was more sensitive than conventional PCR, showing a LoD of 0.01 parasite (p)/μL. Reproducibility and repeatability (precision) were 100% for up to 0.1 p/μL in SYBR™ Green and 1 p/μL in TaqMan™ and conventional PCR.
conclusionReal-time PCR may replace conventional PCR in reference laboratories for P. vivax detection due to its rapidity. The TaqMan™ system is the most indicated when quantification assays are required. Performing tests in triplicate when diagnosing Plasmodium-infected-asymptomatic individuals is recommended to minimise diagnostic errors.
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Registered trials
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