ArticleMethods in molecular biology (Clifton, N.J.)2019
Metabolic Labeling of Cultured Mammalian Cells for Stable Isotope-Resolved Metabolomics: Practical Aspects of Tissue Culture and Sample Extraction.
Article in Methods in molecular biology (Clifton, N.J.), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed.
- Protocol for stable isotope tracing of primary human peripheral blood mononuclear cells.STAR protocols · 2026Article
- Decoding cancer across scales with metabolomics.Nature reviews. Cancer · 2026Review
- Article
- Understanding the Metabolic Effects of Surgically Induced Renal Ischemia in Humans: A Temporal Approach.Metabolites · 2025Article
- Targeting metabolic vulnerability by combining NAMPT inhibitors and disulfiram for treatment of recurrent ovarian cancer.Cell death & disease · 2025Article
- NMR-Based Stable Isotope Tracing of Cancer Metabolism.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Metabolic Pathway Tracing for NADMethods in molecular biology (Clifton, N.J.) · 2025Article
- Differences in glycolytic metabolism between tissue-resident alveolar macrophages and recruited lung macrophages.Frontiers in immunology · 2025Review
- NMR-Based Stable Isotope Tracing of Cancer Metabolism.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Stable Isotope Tracing Analysis in Cancer Research: Advancements and Challenges in Identifying Dysregulated Cancer Metabolism and Treatment Strategies.Metabolites · 2024Review
- Determination of fatty acid uptake and desaturase activity in mammalian cells by NMR-based stable isotope tracing.Analytica chimica acta · 2024Article
- Polarization and β-Glucan Reprogram Immunomodulatory Metabolism in Human Macrophages and Ex Vivo in Human Lung Cancer Tissues.Journal of immunology (Baltimore, Md. : 1950) · 2022Article
- Targeting mutant p53-R248W reactivates WT p53 function and alters the onco-metabolic profile.Frontiers in oncology · 2022Article
- Simple Esterification of [1-ACS chemical biology · 2021Article
- Real-Time insight into in vivo redox status utilizing hyperpolarized [1-Scientific reports · 2021Article
- NMR Methods for Determining Lipid Turnover via Stable Isotope Resolved Metabolomics.Metabolites · 2021Article
- Blockade of 6-phosphogluconate dehydrogenase generates CD8Cell reports · 2021Article
- Metabolomic Approaches to Study Chemical Exposure-Related Metabolism Alterations in Mammalian Cell Cultures.International journal of molecular sciences · 2020Review
- Imaging of glucose metabolism by 13C-MRI distinguishes pancreatic cancer subtypes in mice.eLife · 2019Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Stable isotope-resolved metabolomics (SIRM) methods are used increasingly by cancer researchers to probe metabolic pathways and identify vulnerabilities in cancer cells. Analytical and computational advances are being made constantly, but tissue culture and sample extraction procedures are often variable and not elaborated in the literature. This chapter discusses basic aspects of tissue culture practices as they relate to the use of stable isotope tracers and provides a detailed metabolic labeling and metabolite extraction procedure designed to maximize the amount of information that can be obtained from a single tracer experiment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.