Evidence map›Paper›PMID 30724623›Full record

ArticleJournal of enzyme inhibition and medicinal chemistry2019

An AGT-based protein-tag system for the labelling and surface immobilization of enzymes on E. coli outer membrane.

Rosa Merlo, Sonia Del Prete, Anna Valenti, Rosanna Mattossovich, Vincenzo Carginale, Claudiu T Supuran, Clemente Capasso, Giuseppe Perugino

Open access · goldAbstract read
In one paragraph

Article in Journal of enzyme inhibition and medicinal chemistry, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.4field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 17 citations in OpenAlex.

  1. First thermostable CLIP-Computational and structural biotechnology journal · 2022
    Article
  2. The SNAP-Journal of enzyme inhibition and medicinal chemistry · 2021
    Article
  3. Article
  4. Review
  5. International journal of molecular sciences · 2020
    Review
  6. Article
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Rosa Merloa Department of Biology Agriculture and Food Sciences , Institute of Bioscience and BioResources - National Research Council of Italy , Naples , Italy.
Sonia Del Pretea Department of Biology Agriculture and Food Sciences , Institute of Bioscience and BioResources - National Research Council of Italy , Naples , Italy.
Anna Valentia Department of Biology Agriculture and Food Sciences , Institute of Bioscience and BioResources - National Research Council of Italy , Naples , Italy.
Rosanna Mattossovicha Department of Biology Agriculture and Food Sciences , Institute of Bioscience and BioResources - National Research Council of Italy , Naples , Italy.
Vincenzo Carginalea Department of Biology Agriculture and Food Sciences , Institute of Bioscience and BioResources - National Research Council of Italy , Naples , Italy.
Claudiu T Supuranb Neurofarba Department , University of Florence, Polo Scientifico , Sesto Fiorentino Firenze , Italy.ORCID http://orcid.org/0000-0003-4262-0323
Clemente Capassoa Department of Biology Agriculture and Food Sciences , Institute of Bioscience and BioResources - National Research Council of Italy , Naples , Italy.ORCID http://orcid.org/0000-0003-3314-2411
Giuseppe Peruginoa Department of Biology Agriculture and Food Sciences , Institute of Bioscience and BioResources - National Research Council of Italy , Naples , Italy.
Institute of Biosciences and Bioresources · ITUniversity of Florence · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The use of natural systems, such as outer membrane protein A (OmpA), phosphoporin E (PhoE), ice nucleation protein (INP), etc., has been proved very useful for the surface exposure of proteins on the outer membrane of Gram-negative bacteria. These strategies have the clear advantage of unifying in a one-step the production, the purification and the in vivo immobilisation of proteins/biocatalysts onto a specific biological support. Here, we introduce the novel Anchoring-and-Self-Labelling-protein-tag (ASL

Indexed as

Bacterial Outer Membrane ProteinsEnzymes, ImmobilizedEscherichia coliHumansStaining and LabelingSurface PropertiesTransferasesBacterial Outer Membrane ProteinsEnzymes, ImmobilizedTransferasesCarbonic anhydraseenzyme immobilisation-glycoside hydrolaseice nucleation proteinthermostable

Identifiers

PMID30724623
PMCPMC6366409
OpenAlexW2914364115

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.