ArticleSynthetic and systems biotechnology2019
Miniaturisation of high-throughput plasmid DNA library preparation for next-generation sequencing using multifactorial optimisation.
Article in Synthetic and systems biotechnology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 12 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed.
- Validation of Golden Gate Assemblies Using Highly Multiplexed Nanopore Amplicon Sequencing.Methods in molecular biology (Clifton, N.J.) · 2025Article
- An Automated pre-Dilution Setup for Von Willebrand Factor Activity Assays.Bio-protocol · 2024Article
- DuBA.flow─A Low-Cost, Long-Read Amplicon Sequencing Workflow for the Validation of Synthetic DNA Constructs.ACS synthetic biology · 2024Article
- Complete sequences of pIJ101-based Streptomyces-Escherichia coli shuttle vectors.Access microbiology · 2024Article
- Enabling technology and core theory of synthetic biology.Science China. Life sciences · 2023Review
- Removing the Bottleneck: Introducing cMatch - A Lightweight Tool for Construct-Matching in Synthetic Biology.Frontiers in bioengineering and biotechnology · 2021Article
- Next-Generation Sequencing Based Gut Resistome Profiling of Broiler Chickens Infected with Multidrug-ResistantAnimals : an open access journal from MDPI · 2020Article
- High-throughput Minitaturized RNA-Seq Library Preparation.Journal of biomolecular techniques : JBT · 2020Article
- A specific combination of dual index adaptors decreases the sensitivity of amplicon sequencing with the Illumina platform.DNA research : an international journal for rapid publication of reports on genes and genomes · 2020Article
- A Novel Next-Generation Sequencing and Analysis Platform to Assess the Identity of Recombinant Adeno-Associated Viral Preparations from Viral DNA Extracts.Human gene therapy · 2020Article
- Effects of sample age on data quality from targeted sequencing of museum specimens: what are we capturing in time?BMC genomics · 2020Article
- Highly multiplexed, fast and accurate nanopore sequencing for verification of synthetic DNA constructs and sequence libraries.Synthetic biology (Oxford, England) · 2019Article
Corrections and comments
- Erratum issued
Authors and funding
7 authors.
Funding
Abstract
High-throughput preparation of plasmid DNA libraries for next-generation sequencing (NGS) is an important capability for molecular biology laboratories. In particular, it is an essential quality control (QC) check when large numbers of plasmid variants are being generated. Here, we describe the use of the Design of Experiments (DOE) methodology to optimise the miniaturised preparation of plasmid DNA libraries for NGS, using the Illumina
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.