ArticleNature structural & molecular biology2019
Nascent-protein ubiquitination is required for heat shock-induced gene downregulation in human cells.
Article in Nature structural & molecular biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 54 citations in OpenAlex.
- Principles and mechanisms of plant acclimation to heat stress.Nature reviews. Molecular cell biology · 2026Review
- Oxidative stress triggers RNAPII arrest through PARylation and DNA damage.Nature communications · 2026Article
- Cellular stress triggers degradation and global redistribution of RNAPII.Science advances · 2026Article
- Cytoplasmic mRNA decay by the antiviral nuclease RNase L promotes transcriptional repression.Cell reports · 2026Article
- Dynamic regulation of murine RNA polymerase III transcription during heat shock stress.Genetics · 2025Article
- Gene expression plasticity in response to rapid and extreme elevation changes in Perdix hodgsoniae (Tibetan Partridge).Ornithological applications · 2025Article
- The ubiquitin codes in cellular stress responses.Protein & cell · 2024Review
- Protein quality control: from molecular mechanisms to therapeutic intervention-EMBO workshop, May 21-26 2023, Srebreno, Croatia.Cell stress & chaperones · 2023Review
- Quantitative real-time in-cell imaging reveals heterogeneous clusters of proteins prior to condensation.Nature communications · 2023Article
- The APC/C E3 ligase subunit ANAPC11 mediates FOXO3 protein degradation to promote cell proliferation and lymph node metastasis in urothelial bladder cancer.Cell death & disease · 2023Article
- MAPK/MAK/MRK overlapping kinase (MOK) controls microglial inflammatory/type-I IFN responses via Brd4 and is involved in ALS.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Phase separation in gene transcription control.Acta biochimica et biophysica Sinica · 2023Review
- Current methodologies in protein ubiquitination characterization: from ubiquitinated protein to ubiquitin chain architecture.Cell & bioscience · 2022Review
- Transcriptional lockdown during acute proteotoxic stress.Trends in biochemical sciences · 2022Review
- Cell cycle arrest explains the observed bulk 3D genomic alterations in response to long-term heat shock in K562 cells.Genome research · 2022Article
- DNA damage-induced transcription stress triggers the genome-wide degradation of promoter-bound Pol II.Nature communications · 2022Article
- Heat shock induces premature transcript termination and reconfigures the human transcriptome.Molecular cell · 2022Article
- Adaptive translational pausing is a hallmark of the cellular response to severe environmental stress.Molecular cell · 2021Article
- Primordial super-enhancers: heat shock-induced chromatin organization in yeast.Trends in cell biology · 2021Review
- Improved CRISPR/Cas9 gene editing in primary human myoblasts using low confluency cultures on Matrigel.Skeletal muscle · 2021Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Proteotoxic stress such as heat shock causes heat-shock factor (HSF)-dependent transcriptional upregulation of chaperones. Heat shock also leads to a rapid and reversible downregulation of many genes, a process we term stress-induced transcriptional attenuation (SITA). The mechanism underlying this conserved phenomenon is unknown. Here we report that enhanced recruitment of negative transcription elongation factors to gene promoters in human cell lines induces SITA. A chemical inhibitor screen showed that active translation and protein ubiquitination are required for the response. We further find that proteins translated during heat shock are subjected to ubiquitination and that p38 kinase signaling connects cytosolic translation with gene downregulation. Notably, brain samples of subjects with Huntington's disease also show transcriptional attenuation, which is recapitulated in cellular models of protein aggregation similar to heat shock. Thus our work identifies an HSF-independent mechanism that links nascent-protein ubiquitination to transcriptional downregulation during heat shock, with potential ramifications in neurodegenerative diseases.
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