Evidence map›Paper›PMID 30721669›Full record

ArticleAnalytical biochemistry2019

Surface plasmon resonance and cytotoxicity assays of drug efficacies predicted computationally to inhibit p53/MDM2 interaction.

Xiaoying Wang, Patrycja Magdziarz, Ernest Enriquez, Wang Zhao, Chris Quan, Narek Darabedian, Jamil Momand, Feimeng Zhou

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Analytical biochemistry, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 99% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Xiaoying WangCollege of Chemistry and Chemical Engineering, Central South University, Changsha, Hunan, 410083, PR China.
Patrycja MagdziarzDepartment of Chemistry and Biochemistry, California State University, Los Angeles, Los Angeles, CA, 90032, USA.
Ernest EnriquezDepartment of Chemistry and Biochemistry, California State University, Los Angeles, Los Angeles, CA, 90032, USA.
Wang ZhaoDepartment of Chemistry and Biochemistry, California State University, Los Angeles, Los Angeles, CA, 90032, USA.
Chris QuanDepartment of Chemistry and Biochemistry, California State University, Los Angeles, Los Angeles, CA, 90032, USA.
Narek DarabedianDepartment of Chemistry and Biochemistry, California State University, Los Angeles, Los Angeles, CA, 90032, USA.
Jamil MomandDepartment of Chemistry and Biochemistry, California State University, Los Angeles, Los Angeles, CA, 90032, USA. Electronic address: JMomand@calstatela.edu.
Feimeng ZhouDepartment of Chemistry and Biochemistry, California State University, Los Angeles, Los Angeles, CA, 90032, USA. Electronic address: fzhou@calstatela.edu.
California State University Los Angeles · USCentral South University · CN

Funding

Macromolecular Interactions of t-Darpp and Darpp-32R01GM105898 · NIGMS · CALIFORNIA STATE UNIVERSITY LOS ANGELES · PI MOMAND, JAMIL A, ZHOU, FEIMENG · 2014 to 2017
$1.3M
NIGMS NIH HHS R01 GM105898
6 · The paper itself

Abstract

Docking on the p53-binding site of murine double minute 2 (MDM2) by small molecules restores p53's tumor-suppressor function. We previously assessed 3244 FDA-approved drugs via "computational conformer selection" for inhibiting MDM2 and p53 interaction. Here, we developed a surface plasmon resonance method to experimentally confirm the inhibitory effects of the known MDM2 inhibitor, nutlin-3a, and two drug candidates predicted by our computational method. This p53/MDM2 interaction displayed a dosage-dependent weakening when MDM2 is pre-mixed with drug candidates. The inhibition efficiency order is nutlin-3a (IC

Indexed as

Surface Plasmon ResonanceBepridilCell Line, TumorHumansImidazolesPiperazinesProtein BindingProto-Oncogene Proteins c-mdm2Small Molecule LibrariesTumor Suppressor Protein p53BepridilImidazolesnutlin 3PiperazinesProto-Oncogene Proteins c-mdm2Small Molecule LibrariesTumor Suppressor Protein p53Drug screeningInhibitory effectsMDM2p53Repurposing of existing drugs

Identifiers

PMID30721669
PMCPMC6942878
OpenAlexW2913710737

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.