ArticleMolecular biology reports2019
ELEquant: a developmental framework and validation of forensic and conservation real-time PCR assays.
Article in Molecular biology reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Development and validation of simultaneous identification of 26 mammalian and poultry species by a multiplex assay.International journal of legal medicine · 2022Article
- Discrimination of highly degraded, aged Asian and African elephant ivory using denaturing gradient gel electrophoresis (DGGE).International journal of legal medicine · 2021Article
- Development of a multiplex, PCR-based genotyping assay for African and Asian elephants for forensic purposes.International journal of legal medicine · 2020Article
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Authors and funding
4 authors.
Funding
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Abstract
A framework for the development and validation of a qPCR assay for species identification and DNA quantification for conservation and forensic purposes is presented. Elephants are commonly poached for their ivory tusks, which is the primary driving force behind their endangered status. In addition to poaching and trade, habitat loss due to logging and mining has also resulted in loss of elephants. Crimes against animals can be deterred and/or further prosecution sought through testing with forensic genetic techniques. The creation of novel genetic assays can greatly impact wildlife forensic science investigations in identifying the species. Molecular genetic techniques can help enforce conservation efforts; however, they must be properly developed and validated to be of evidentiary quality for court systems. African and Asian elephant buccal cells were used as model in this work. The assay provides a method to differentiate biological fluids of both genera of elephants simultaneously. It can be used for identification of elephant derived products and presents valuable quantification for optimized further testing, such as microsatellite detection.
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Registered trials
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