ArticleScientific data2019
The effect of 16S rRNA region choice on bacterial community metabarcoding results.
Article in Scientific data, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 182 papers, 2 of them syntheses that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
182 citing papers in PubMed, 2 syntheses or guidelines pooled it.
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- Comparative analysis of root microbiomes in four Swertia species from Taiwan.Journal of plant research · 2026Article
- The role of the microbiota in hematological malignancies: A narrative review of mechanisms and therapeutic potential.New microbes and new infections · 2026Review
- Can the leafhopper microbiome unlock new strategies for its control?Journal of economic entomology · 2026Review
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- Isothermal assay targeting the V1-V2 region of the 16S rRNA gene for rapid screening ofInternational dairy journal · 2026Article
- Microbial Distributions Across Wide-Ranging Temperature Gradients of Hot Springs in Thailand: A Review of 35 Years of Research on Hot Spring-Associated Microorganisms.Environmental microbiology reports · 2026Review
- Bioremediation of Saline-Alkali Soil Using a Waste Biomass-Functional Microorganism Composite Amendment and Preliminary Multi-Crop Field Validation.Microorganisms · 2026Article
- Advances in Shotgun Metagenomics for Cheese Microbiology: From Microbial Dynamics to Functional Insights.Foods (Basel, Switzerland) · 2026Review
- Synthetic toehold biosensors for detectingFrontiers in microbiology · 2026Article
- Significant impact of threshold adjustments on microbiome characterization following Nanopore sequencing.ISME communications · 2026Article
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- Coexistence of methanogenesis and sulfate reduction in a sulfate-adapted enrichment culture from an oil reservoir.Applied and environmental microbiology · 2025Article
- Gut-ovary axis and multiomic insights into PCOS in a DHEA-induced rat model.Scientific reports · 2025Article
- Isolation and characterization of multi-drug-resistant Microbacterium esteraromaticum bacteriophage: assessment of antibacterial efficacy and genomic insights.International microbiology : the official journal of the Spanish Society for Microbiology · 2025Article
- Disentangling Gut Bacterial Community Patterns inInsects · 2025Article
- Ecological Health and Freshwater Pathogen Using eDNA Metabarcoding: A Preliminary Assessment for Environmental Surveillance Development in Malaysia.Microorganisms · 2025Article
122 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issued
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this work, we compare the resolution of V2-V3 and V3-V4 16S rRNA regions for the purposes of estimating microbial community diversity using paired-end Illumina MiSeq reads, and show that the fragment, including V2 and V3 regions, has higher resolution for lower-rank taxa (genera and species). It allows for a more precise distance-based clustering of reads into species-level OTUs. Statistically convergent estimates of the diversity of major species (defined as those that together are covered by 95% of reads) can be achieved at the sample sizes of 10000 to 15000 reads. The relative error of the Shannon index estimate for this condition is lower than 4%.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.