ArticleNature microbiology2019
In plaque-mass spectrometry imaging of a bloom-forming alga during viral infection reveals a metabolic shift towards odd-chain fatty acid lipids.
Article in Nature microbiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 69 citations in OpenAlex.
- MALDI Tandem Mass Spectrometry for Colony-Based Dereplication of Natural Products.bioRxiv : the preprint server for biology · 2026Article
- Applications and technical challenges of MALDI mass spectrometry imaging in microbial ecosystems and metabolic network research.Archives of microbiology · 2026Review
- Preventive and Ameliorative Effects of Alanine Supplementation on High-Fat Diet-Induced Obesity and Metabolic Dysfunction via the Gut-Liver-Adipose Axis in C57BL/6J Mice.Foods (Basel, Switzerland) · 2026Article
- Diving into the hidden viral world of marine protists.Journal of virology · 2026Review
- Methodologies for Assessing Chemical Toxicity to Aquatic Microorganisms: A Comparative Review.Molecules (Basel, Switzerland) · 2026Review
- From microbes to molecules: unveiling host-microbe interactions with spatial metabolomics.Molecular systems biology · 2025Article
- Spatial Metabolomics and Its Application in Plant Research.International journal of molecular sciences · 2025Review
- Soil viral-host interactions regulate microplastic-dependent carbon storage.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Imaging Mass Spectrometry and Genome Mining Reveal Antimicrobial Peptides of NovelFoods (Basel, Switzerland) · 2024Article
- Seeing is Believing: Developing Multimodal Metabolic Insights at the Molecular Level.ACS central science · 2024Review
- The consequences of viral infection on protists.Communications biology · 2024Review
- Algal blooms in the ocean: hot spots for chemically mediated microbial interactions.Nature reviews. Microbiology · 2024Review
- Production of structurally diverse sphingolipids by anaerobic marine bacteria in the euxinic Black Sea water column.The ISME journal · 2024Article
- Daily turnover of active giant virus infection during algal blooms revealed by single-cell transcriptomics.Science advances · 2023Article
- Virologs, viral mimicry, and virocell metabolism: the expanding scale of cellular functions encoded in the complex genomes of giant viruses.FEMS microbiology reviews · 2023Article
- Identification and Verification of Candidate miRNA Biomarkers with Application to Infection withGenes · 2023Article
- Lipid biomarkers for algal resistance to viral infection in the ocean.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Viral infection in the ocean-A journey across scales.PLoS biology · 2023Article
- Review
- Giant virus biology and diversity in the era of genome-resolved metagenomics.Nature reviews. Microbiology · 2022Review
Corrections and comments
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Authors and funding
12 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Tapping into the metabolic crosstalk between a host and its virus can reveal unique strategies employed during infection. Viral infection is a dynamic process that generates an evolving metabolic landscape. Gaining a continuous view into the infection process is highly challenging and is limited by current metabolomics approaches, which typically measure the average of the entire population at various stages of infection. Here, we took an innovative approach to study the metabolic basis of host-virus interactions between the bloom-forming alga Emiliania huxleyi and its specific virus. We combined a classical method in virology, the plaque assay, with advanced mass spectrometry imaging (MSI), an approach we termed 'in plaque-MSI'. Taking advantage of the spatial characteristics of the plaque, we mapped the metabolic landscape induced during infection in a high spatiotemporal resolution, unfolding the infection process in a continuous manner. Further unsupervised spatially aware clustering, combined with known lipid biomarkers, revealed a systematic metabolic shift during infection towards lipids containing the odd-chain fatty acid pentadecanoic acid (C15:0). Applying 'in plaque-MSI' may facilitate the discovery of bioactive compounds that mediate the chemical arms race of host-virus interactions in diverse model systems.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.