ArticleScientific reports2019
A transcription factor PU.1 is critical for Ccl22 gene expression in dendritic cells and macrophages.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 27 citations in OpenAlex.
- Association of PCSK9 and CCL22 gene polymorphisms with myocardial infarction in a South Indian population.Functional & integrative genomics · 2026Article
- CCR-CCL axes as key upstream influencers of pancreatic ductal adenocarcinoma: CCR2-CCL2, CCR5-CCL5, CCR4-CCL17/22, CCR6-CCL20, CCR7-CCL19/21.Frontiers in immunology · 2026Review
- IRF4-regulated transcriptional and functional heterogeneity of lung-resident CD11b+ cDC2 subsets during influenza virus infection.Journal of immunology (Baltimore, Md. : 1950) · 2025Article
- Gold and Silver Nanoparticles Efficiently Modulate the Crosstalk Between Macrophages and Cancer Cells.International journal of nanomedicine · 2025Article
- Review
- ETS Transcription Factors in Immune Cells and Immune-Related Diseases.International journal of molecular sciences · 2024Review
- Ectopic PU.1 Expression Provides Chimeric Antigen Receptor (CAR) T Cells with Innate Cell Capacities Including IFN-β Release.Cancers · 2024Article
- Chemokines and Cytokines in Immunotherapy of Melanoma and Other Tumors: From Biomarkers to Therapeutic Targets.International journal of molecular sciences · 2024Review
- Transcriptional and functional remodeling of lung-resident T cells and macrophages by Simian varicella virus infection.Frontiers in immunology · 2024Article
- Increase of the T-reg-recruiting chemokine CCL22 expression in a progressive course of cervical dysplasia.Journal of cancer research and clinical oncology · 2023Article
- Modulation of mouse laryngeal inflammatory and immune cell responses by low and high doses of mainstream cigarette smoke.Scientific reports · 2022Article
- Identifying patterns of immune related cells and genes in the peripheral blood of acute myocardial infarction patients using a small cohort.Journal of translational medicine · 2022Article
- Digital Cell Atlas of Mouse Uterus: From Regenerative Stage to Maturational Stage.Frontiers in genetics · 2022Article
- ATP6V0d2 Suppresses Alveoli Macrophage Alternative Polarization and Allergic Asthma via Degradation of PU.1.Allergy, asthma & immunology research · 2021Article
- PU.1 and IRF8 Modulate Activation of NLRP3 InflammasomeFrontiers in immunology · 2021Article
- Lung Function in African American Children with Asthma Is Associated with Novel Regulatory Variants of the KIT LigandGenetics · 2020Article
- PU.1 regulates Ccr7 gene expression by binding to its promoter in naïve CD4FEBS open bio · 2020Article
- Transcriptional, Epigenetic and Metabolic Programming of Tumor-Associated Macrophages.Cancers · 2020Review
- Derangement of Metabolic and Lysosomal Gene Profiles in Response to Dexamethasone Treatment in Sarcoidosis.Frontiers in immunology · 2020Article
- Weighted Gene Co-Expression Network Analysis Identifies Critical Genes in the Development of Heart Failure After Acute Myocardial Infarction.Frontiers in genetics · 2019Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The chemokine CCL22 is predominantly produced by dendritic cells (DCs) and macrophages. CCL22 acts on CCR4-expressing cells including Th2 and Treg. Although a correlation between the CCL22-CCR4 axis and allergic diseases has been established, the mechanism of monocyte lineage-specific Ccl22 gene expression is largely unknown. In the current study, we investigated transcriptional regulation of the Ccl22 gene in DCs and macrophages. Using reporter assays, we identified the critical cis-enhancing elements at 21/-18 and -10/-4 in the Ccl22 promoter. Electrophoretic mobility shift assays proved that transcription factor PU.1 directly binds to the cis-elements. Knockdown of PU.1 markedly decreased Ccl22 expression in bone marrow-derived DCs (BMDCs) and BM macrophages (BMDMs). Chromatin immunoprecipitation assays revealed that PU.1 bound to the Ccl22 promoter in not only BMDCs and BMDMs, but also splenic DCs and peritoneal macrophages. LPS stimulation increased the amount of PU.1 recruited to the promoter, accompanied by upregulation of the Ccl22 mRNA level, which was diminished by Spi1 knockdown. We identified similar cis-elements on the human CCL22 promoter, which were bound with PU.1 in human monocytes. Taken together, these findings indicate that PU.1 transactivates the Ccl22 gene in DCs and macrophages by directly binding to the two elements in the promoter.
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