ArticleScientific reports2019
A continuous fluorescence assay for simple quantification of bile salt hydrolase activity in the gut microbiome.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 31 citations in OpenAlex.
- A Screening Assay for Bile Acid-Transforming Microorganisms Using Engineered Bacterial Biosensors.Biosensors · 2025Article
- Intestinal microbiota composition and bile salt hydrolase activity in fast and slow growing broiler chickens: implications for growth performance and production efficiency.Journal of animal science and biotechnology · 2025Article
- Variability of Bile Baseline Excitation-emission Fluorescence of Two Tropical Freshwater Fish Species.Journal of fluorescence · 2025Article
- Leucine aminopeptidase LyLAP enables lysosomal degradation of membrane proteins.Science (New York, N.Y.) · 2025Article
- Chemoproteomic profiling of substrate specificity in gut microbiota-associated bile salt hydrolases.Cell chemical biology · 2025Article
- Bile Salt Hydrolase Activity-Based Probes for Monitoring Gut Microbial Bile Acid Metabolism.Chembiochem : a European journal of chemical biology · 2024Article
- Chemoproteomic profiling of substrate specificity in gut microbiota-associated bile salt hydrolases.bioRxiv : the preprint server for biology · 2024Article
- Profiling rhythmicity of bile salt hydrolase activity in the gut lumen with a rapid fluorescence assay.Organic & biomolecular chemistry · 2023Article
- BSH-TRAP: Bile salt hydrolase tagging and retrieval with activity-based probes.Methods in enzymology · 2022Article
- In vitro properties of potential probiotic lactic acid bacteria originating from Ghanaian indigenous fermented milk products.World journal of microbiology & biotechnology · 2021Article
- Noninvasive imaging and quantification of bile salt hydrolase activity: From bacteria to humans.Science advances · 2021Article
- Targeting the Liver-Brain Axis with Hop-Derived Flavonoids Improves Lipid Metabolism and Cognitive Performance in Mice.Molecular nutrition & food research · 2020Article
- Chemoproteomic Profiling of Gut Microbiota-Associated Bile Salt Hydrolase Activity.ACS central science · 2019Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The microbiota of the mammalian gut plays a dynamic role in controlling host physiology. The effect of gut microbiota activity on host health is particularly evident in the case of bile homeostasis. Bile is produced by the host and is modified by the gut microbiota, which impacts the net hydrophobicity of the total bile acid pool, and also modulates host signaling pathways. A key mechanism by which the microbiota modify bile is through deconjugation of bile salts through bile salt hydrolase (BSH) enzymatic activity, which is postulated to be a prerequisite for all further microbial metabolism. BSH activity in the gut is largely considered to be beneficial for the host, and genes encoding BSHs are found in the genomes of many taxa found in over-the-counter probiotics. Despite the therapeutic relevance of this enzyme, there is no sensitive and simple assay for continuous monitoring of BSH activity, and there are no non-destructive means of characterizing its activity in whole cell or microbial community samples. Herein, we describe a continuous fluorescence assay that can be used for characterization of BSH activity with purified protein, cell lysates, whole cells, and in human gut microbiome samples. The method is a "turn-on" reporter strategy, which employs synthetic substrates that yield a fluorescent product upon BSH-dependent turnover. This assay is used to show the first in vivo characterization of BSH activity. We also demonstrate continuous, non-destructive quantification of BSH activity in a human fecal microbiome sample containing recombinant BSH.
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