Evidence map›Paper›PMID 30718604›Full record

ArticleScientific reports2019

Diversity and environmental distribution of the cosmopolitan endosymbiont "Candidatus Megaira".

Olivia Lanzoni, Elena Sabaneyeva, Letizia Modeo, Michele Castelli, Natalia Lebedeva, Franco Verni, Martina Schrallhammer, Alexey Potekhin, Giulio Petroni

Open access · goldAbstract read
In one paragraph

Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.

0numbers the graph read from it
0cells of the map it votes in
27citing papers in PubMed
3.1field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

27 citing papers in PubMed, 52 citations in OpenAlex.

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  16. The "Other"Applied and environmental microbiology · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 3 countries.

Olivia LanzoniDepartment of Biology, University of Pisa, Pisa, Italy.
Elena SabaneyevaFaculty of Biology, Saint Petersburg State University, Saint Petersburg, Russia.
Letizia ModeoDepartment of Biology, University of Pisa, Pisa, Italy.
Michele CastelliCentro Romeo ed Enrica Invernizzi Ricerca Pediatrica, Dipartimento di Bioscienze, Università degli studi di Milano, Milan, Italy.
Natalia LebedevaCore Facilities Centre "Culture Collections of Microorganisms", Saint Petersburg State University, Saint Petersburg, Russia.
Franco VerniDepartment of Biology, University of Pisa, Pisa, Italy.
Martina SchrallhammerMicrobiology, University of Freiburg, Freiburg, Germany.
Alexey PotekhinFaculty of Biology, Saint Petersburg State University, Saint Petersburg, Russia.
Giulio PetroniDepartment of Biology, University of Pisa, Pisa, Italy. giulio.petroni@unipi.it.ORCID 0000-0001-9572-9897
University of Pisa · ITSt Petersburg University · RUFoundation Jewish Contemporary Documentation Center · ITUniversity of Freiburg · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Members of the order Rickettsiales are often found in association with ciliated protists. An interesting case is the bacterial endosymbiont "Candidatus Megaira", which is phylogenetically closely related to the pathogen Rickettsia. "Candidatus Megaira" was first described as an intracellular bacterium in several ciliate species. Since then it has been found in association with diverse evolutionary distantly-related hosts, among them other unicellular eukaryotes, and also algae, and metazoa, such as cnidarians. We provide the characterization of several new strains of the type species "Candidatus Megaira polyxenophila", and the multidisciplinary description of a novel species, "Candidatus Megaira venefica", presenting peculiar features, which highlight the diversity and variability of these widespread bacterial endosymbionts. Screening of the 16S rRNA gene short amplicon database and phylogenetic analysis of 16S rRNA gene hypervariable regions revealed the presence of further hidden lineages, and provided hints on the possibility that these bacteria may be horizontally transmitted among aquatic protists and metazoa. The phylogenetic reconstruction supports the existence of at least five different separate species-level clades of "Candidatus Megaira", and we designed a set of specific probes allowing easy recognition of the four major clades of the genus.

Indexed as

Genetic VariationSymbiosisAquatic OrganismsCiliophoraDNA, BacterialDNA, RibosomalPhylogenyRickettsiaceaeRNA, Ribosomal, 16SSequence Analysis, DNADNA, BacterialDNA, RibosomalRNA, Ribosomal, 16S

Identifiers

PMID30718604
PMCPMC6362216
OpenAlexW2914627959

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.