ArticleProceedings of the National Academy of Sciences of the United States of America2019
Mature vessel networks in engineered tissue promote graft-host anastomosis and prevent graft thrombosis.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers, 1 of them a synthesis that pooled it.
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Who cites it
58 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Recent advancements and future requirements in vascularization of cortical organoids.Frontiers in bioengineering and biotechnology · 2022Pooled it
- Prevascularized grafts with spatially organized MSC spheroids to accelerate therapeutic angiogenesis in ischemic disease.Angiogenesis · 2026Article
- Active Microrheology Reveals Distinct ECM Mechanical Signatures Induced by Stromal Cells of Different Tissue Origins during Vascular Morphogenesis.ACS biomaterials science & engineering · 2026Article
- Electrical Stimulation Directs Formation of Perfused Vasculature in Engineered Tissues.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- A 3D prevascularized calcium phosphate cement scaffold for accelerated alveolar bone regeneration and angiogenesis in rats.BMC oral health · 2026Article
- Synthetic control of implanted engineered liver tissue growth.Science advances · 2026Article
- Generation of Fibrin-Based Aortic Vessels with Layer-Specific Cell Architecture Under Pulsatile Perfusion in a Clinical Organ Care System.Annals of biomedical engineering · 2026Article
- The Effect of Mechanical Loading on Sprouting Angiogenesis from Engineered Macro-vessel Model.Small methods · 2026Article
- Biomimetic hydrogel design strategies for vascular grafts and vascularized tissue constructs.Frontiers in bioengineering and biotechnology · 2026Review
- EphrinB2 in dental pulp stem cells promotes endothelial cells forming capillary-like cords in a 3D bioprinted hydrogel construct.BMC molecular and cell biology · 2025Article
- Nanosilicates promote angiogenesis through activation of ROS-mediated WNT/β-catenin pathway.Science advances · 2025Article
- Activin a regulates vascular formation and stabilization in direct coculture of dental pulp stem cells and endothelial cells.International endodontic journal · 2025Article
- Semi-synthetic fibrous fibrin composites promote 3D microvascular assembly, survival, and host integration of endothelial cells without mesenchymal cell support.Bioactive materials · 2025Article
- Semaphorin-4D signaling in recruiting dental stem cells for vascular stabilization.Stem cell research & therapy · 2025Article
- Sacrificial Templating for Accelerating Clinical Translation of Engineered Organs.ACS biomaterials science & engineering · 2025Review
- Bioprinting of Aptamer-Based Programmable Bioinks to Modulate Multiscale Microvascular Morphogenesis in 4D.Advanced healthcare materials · 2025Article
- Multiscale Imaging to Monitor Functional SHED-Supported Engineered Vessels.Journal of dental research · 2024Article
- Bioengineering vascularization.Development (Cambridge, England) · 2024Review
- Placenta-Derived Mesenchymal Stromal-Like Cells Promote 3D-Engineered Muscle Tissue Differentiation and Vessel Network Maturation.Small science · 2024Article
- Applications, challenges, and prospects of induced pluripotent stem cells for vascular disease.Molecules and cells · 2024Review
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Graft vascularization remains one of the most critical challenges facing tissue-engineering experts in their attempt to create thick transplantable tissues and organs. In vitro prevascularization of engineered tissues has been suggested to promote rapid anastomosis between the graft and host vasculatures; however, thrombotic events have been reported upon graft implantation. Here, we aimed to determine whether in vitro vessel maturation in transplantable grafts can accelerate vascular integration and graft perfusion and prevent thrombotic events in the grafts. To this end, endothelial cells and fibroblasts were cocultured on 3D scaffolds for 1, 7, or 14 d to form vasculature with different maturation degrees. Monitoring graft-host interactions postimplantation demonstrated that the 14-d in vitro-cultured grafts, bearing more mature and complex vessel networks as indicated by elongated and branched vessel structures, had increased graft-host vessel anastomosis; host vessel penetration into the graft increased approximately eightfold, and graft perfusion increased sixfold. The presence of developed vessel networks prevented clot accumulation in the grafts. Conversely, short-term cultured constructs demonstrated poor vascularization and increased thrombus formation. Elevated expression levels of coagulation factors, von Willebrand factor (vWF), and tissue factor (TF), were demonstrated in constructs bearing less mature vasculature. To conclude, these findings demonstrate the importance of establishing mature and complex vessel networks in engineered tissues before implantation to promote anastomosis with the host and accelerate graft perfusion.
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