ArticleBMC infectious diseases2019
Development of a lateral flow recombinase polymerase amplification assay for rapid and visual detection of Cryptococcus neoformans/C. gattii in cerebral spinal fluid.
Article in BMC infectious diseases, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Paper-Based Microfluidic Chips for At-Home Point-of-Care Nucleic Acid Testing: Applications and Challenges.Diagnostics (Basel, Switzerland) · 2026Review
- Rapid and simultaneous detection ofFrontiers in cellular and infection microbiology · 2026Article
- Article
- Recombinase polymerase amplification combined with lateral flow dipstick assay for rapid visual detection ofHeliyon · 2024Article
- Development of Multienzyme Isothermal Rapid Amplification (MIRA) Combined with Lateral-Flow Dipstick (LFD) Assay to Detect Species-SpecificPathogens (Basel, Switzerland) · 2024Article
- Application of recombinase polymerase amplification with lateral flow assay to pathogen point-of-care diagnosis.Frontiers in cellular and infection microbiology · 2024Review
- Comparative analysis of diagnostic methods for the detection of Cryptococcus neoformans meningitis.PLoS neglected tropical diseases · 2023Article
- Rapid detection of Mucorales based on recombinase polymerase amplification and real-time PCR.Frontiers in microbiology · 2023Article
- What's New inJournal of fungi (Basel, Switzerland) · 2022Review
- From saliva to SNP: non-invasive, point-of-care genotyping for precision medicine applications using recombinase polymerase amplification and giant magnetoresistive nanosensors.Lab on a chip · 2022Article
- Recent advances in recombinase polymerase amplification: Principle, advantages, disadvantages and applications.Frontiers in cellular and infection microbiology · 2022Review
- Rapid Detection ofFrontiers in cellular and infection microbiology · 2022Article
- Evaluation of the Performance of a Multiplex Real-Time PCR Assay for the Identification ofInfection and drug resistance · 2022Article
- Recent advances in gold nanoparticle-based lateral flow immunoassay for the detection of bacterial infection.Archives of microbiology · 2021Review
- Rapid Detection of Pathogens in Wound Exudate via Nucleic Acid Lateral Flow Immunoassay.Biosensors · 2021Article
- Bushmeat Species Identification: Recombinase Polymerase Amplification (RPA) Combined with Lateral Flow (LF) Strip for Identification of Formosan Reeves' Muntjac (Animals : an open access journal from MDPI · 2021Article
- Rapid Detection ofFrontiers in cellular and infection microbiology · 2021Article
- Development and Application of Rapid Clinical Visualization Molecular Diagnostic Technology forFrontiers in cellular and infection microbiology · 2021Article
- Preliminary Evaluation of Rapid Visual Identification ofFrontiers in cellular and infection microbiology · 2021Article
- Point-of-Care Strategies for Detection of Waterborne Pathogens.Sensors (Basel, Switzerland) · 2019Review
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7 authors.
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Abstract
backgroundFor definitive diagnosis of cryptococcal meningitis, Cryptococcus neoformans and/or C. gattii must be identified within cerebral spinal fluid from the patients. The traditional methods for detecting Cryptococcus spp. such as India ink staining and culture are not ideal. Although sensitive and specific enough, detection of cryptococcal antigen polysaccharide has a high dose hook effect. Therefore, the aim of this study was to introduce a new rapid and simple detection method of Cryptococcus neoformans and C. gattii in cerebral spinal fluid.
methodsThe lateral flow strips combined with recombinase polymerase amplification (LF-RPA) assay was constructed to detect the specific DNA sequences of C. neoformans and C. gattii. The detection limit was evaluated using serial dilutions of C. neoformans and C. gattii genomic DNA. The specificity was assessed by excessive amount of other pathogens genomic DNA. The optimal detection time and amplification temperature were also analyzed. The diagnostic parameters were first calculated using 114 clinical specimens and then compared with that of other diagnostic method. A brief analysis and comparison of different DNA extraction methods was discussed, too.
resultsThe LF-RPA assay could detect 0.64 pg of genomic DNA of C. neoformans per reaction within 10 min and was highly specific for Cryptococcus spp.. The system could work well at a wide range of temperature from 25 to 45 °C. The overall sensitivity and specificity were 95.2 and 95.8% respectively. As amplification template for LF-RPA assay, both cell lysates and genomic DNA produce similar experimental results.
conclusionsThe LF-RPA system described here is shown to be a sensitive and specific method for the visible, rapid, and accurate detection of Cryptococcus spp. in cerebral spinal fluid and might be useful for clinical preliminary screening of cryptococcal meningitis.
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