Evidence map›Paper›PMID 30717310›Full record

ArticleCells2019

CRISPR/Cas9-Mediated Hitchhike Expression of Functional shRNAs at the Porcine

Chao Lu, Daxin Pang, Mengjing Li, Hongming Yuan, Tingting Yu, Peixuan Huang, Jianing Li, Xue Chen, Huping Jiao, Zicong Xie and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cells, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
0.6field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 16 citations in OpenAlex.

  1. Molecular breeding of pigs in the genome editing era.Genetics, selection, evolution : GSE · 2025
    Review
  2. Article
  3. Review
  4. Review
  5. Review
  6. Article
  7. Review
  8. Article
  9. Review
  10. Classical Swine Fever in China-An Update Minireview.Frontiers in veterinary science · 2019
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 1 institution in 1 country.

Chao LuJilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun 130062, China. swjs092408147@163.com.ORCID 0000-0001-7468-065X
Daxin PangJilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun 130062, China. pdx@jlu.edu.cn.
Mengjing LiJilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun 130062, China. mjli18@mails.jlu.edu.cn.
Hongming YuanJilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun 130062, China. yuanhm17@mails.jlu.edu.cn.
Tingting YuJilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun 130062, China. ttingyu123@163.com.
Peixuan HuangJilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun 130062, China. huangpx16@mails.jlu.edu.cn.
Jianing LiJilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun 130062, China. cool_lijianing@163.com.
Xue ChenJilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun 130062, China. snow1213dawei@163.com.
Huping JiaoJilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun 130062, China. jiaohp@jlu.edu.cn.
Zicong XieJilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun 130062, China. wx11196@163.com.
Hongsheng OuyangJilin Provincial Key Laboratory of Animal Embryo Engineering, College of Animal Sciences, Jilin University, Changchun 130062, China. ouyh@jlu.edu.cn.
Jilin University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Successful RNAi applications depend on strategies allowing stable and persistent expression of minimal gene silencing triggers without perturbing endogenous gene expression. In this study, we proposed an endogenous microRNA (miRNA) cluster as a novel integration site for small hairpin RNAs (shRNAs). We successfully integrated exogenous shRNAs at the porcine

Indexed as

AnimalsAnimals, Genetically ModifiedBase SequenceBlastocystCell LineCRISPR-Associated Protein 9CRISPR-Cas SystemsFetusFibroblastsGenes, ReporterGenomeGreen Fluorescent ProteinsMicroRNAsRNA, Guide, CRISPR-Cas SystemsRNA, Small InterferingSwineCRISPR-Associated Protein 9enhanced green fluorescent proteinGreen Fluorescent ProteinsMicroRNAsRNA, Guide, CRISPR-Cas SystemsRNA, Small Interferingclassical swine fever virusCRISPR/Cas9miRNA-17-92 clusterRNAishRNA

Identifiers

PMID30717310
PMCPMC6406430
OpenAlexW2912233393

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.