ArticlePlant cell reports2019
CRISPR/Cas9 mutations in the rice Waxy/GBSSI gene induce allele-specific and zygosity-dependent feedback effects on endosperm starch biosynthesis.
Article in Plant cell reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 59 citations in OpenAlex.
- Unlocking Low-glycemic Potential in Rice: Genetic Insights and Breeding Strategies.Plant foods for human nutrition (Dordrecht, Netherlands) · 2026Review
- Modification of starch synthesis in food crops using CRISPR/Cas9 gene editing technology for changing climate.Stress biology · 2026Review
- A distal regulatory element regulates Wx gene expression and the amylose content in rice.Frontiers in plant science · 2025Article
- Unveiling distinct storage composition and starch properties in developing indica rice grains via transcriptional profiling and enzymatic activity analysis.Computational and structural biotechnology journal · 2025Article
- CRISPR/Cas9-mediated enhancement of semi-dwarf glutinous traits in elite Xiangdaowan rice (Frontiers in plant science · 2024Article
- TaTPP-7A positively feedback regulates grain filling and wheat grain yield through T6P-SnRK1 signalling pathway and sugar-ABA interaction.Plant biotechnology journal · 2023Article
- Enhancing the quality of staple food crops through CRISPR/Cas-mediated site-directed mutagenesis.Planta · 2023Review
- Genetic Engineering of Starch Biosynthesis in Maize Seeds for Efficient Enzymatic Digestion of Starch during Bioethanol Production.International journal of molecular sciences · 2023Review
- Reverse genetic approaches for breeding nutrient-rich and climate-resilient cereal and food legume crops.Heredity · 2022Article
- Genome-wide association mapping and genomic prediction of yield-related traits and starch pasting properties in cassava.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
- Assessment of the Characteristics of Waxy Rice Mutants Generated by CRISPR/Cas9.Frontiers in plant science · 2022Article
- Genome editing in cereal crops: an overview.Transgenic research · 2021Review
- Fruit crops in the era of genome editing: closing the regulatory gap.Plant cell reports · 2021Review
- Application of CRISPR/Cas9 in Crop Quality Improvement.International journal of molecular sciences · 2021Review
- Target-specific mutations efficiency at multiple loci of CRISPR/Cas9 system using one sgRNA in soybean.Transgenic research · 2021Article
- Re-examination of theFrontiers in plant science · 2021Article
- TargetingFrontiers in plant science · 2021Review
- Inactivation of rice starch branching enzyme IIb triggers broad and unexpected changes in metabolism by transcriptional reprogramming.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Comparative transcriptome analysis between inbred and hybrids reveals molecular insights into yield heterosis of upland cotton.BMC plant biology · 2020Article
- CRISPR-associated nucleases: the Dawn of a new age of efficient crop improvement.Transgenic research · 2020Review
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Authors and funding
14 authors at 3 institutions in 1 country.
Funding
Abstract
key messageInduced mutations in the waxy locus in rice endosperm did not abolish GBSS activity completely. Compensatory mechanisms in endosperm and leaves caused a major reprogramming of the starch biosynthetic machinery. The mutation of genes in the starch biosynthesis pathway has a profound effect on starch quality and quantity and is an important target for plant breeders. Mutations in endosperm starch biosynthetic genes may impact starch metabolism in vegetative tissues such as leaves in unexpected ways due to the complex feedback mechanisms regulating the pathway. Surprisingly this aspect of global starch metabolism has received little attention. We used CRISPR/Cas9 to introduce mutations affecting the Waxy (Wx) locus encoding granule-bound starch synthase I (GBSSI) in rice endosperm. Our specific objective was to develop a mechanistic understanding of how the endogenous starch biosynthetic machinery might be affected at the transcriptional level following the targeted knock out of GBSSI in the endosperm. We found that the mutations reduced but did not abolish GBSS activity in seeds due to partial compensation caused by the upregulation of GBSSII. The GBSS activity in the mutants was 61-71% of wild-type levels, similarly to two irradiation mutants, but the amylose content declined to 8-12% in heterozygous seeds and to as low as 5% in homozygous seeds, accompanied by abnormal cellular organization in the aleurone layer and amorphous starch grain structures. Expression of many other starch biosynthetic genes was modulated in seeds and leaves. This modulation of gene expression resulted in changes in AGPase and sucrose synthase activity that explained the corresponding levels of starch and soluble sugars.
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