ArticleJournal of experimental botany2019
Embryogenic competence of microspores is associated with their ability to form a callosic, osmoprotective subintinal layer.
Article in Journal of experimental botany, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 29 citations in OpenAlex.
- From pollen precursor to totipotent embryo: a model system for plant cell developmental plasticity.Plant cell reports · 2026Review
- Recent advances in generation of doubled haploid plants for genetic improvement in solanaceous vegetable crops.Planta · 2026Review
- Transcriptomic analyses reveal regulatory plasticity and metabolic reprogramming underlying genotype-specific microspore embryogenesis in wheat.Plant cell reports · 2026Article
- Unresolved ER stress restricts in vitro plant cell totipotency.Plant cell reports · 2025Article
- Modulation of calcium, callose synthesis, membrane permeability and pectin methyl-esterase activity affect cell wall composition and embryo yield during Brassica napus microspore embryogenesis.Annals of botany · 2025Article
- Calcium levels modulate embryo yield inFrontiers in plant science · 2024Article
- The Highly EmbryogenicPlants (Basel, Switzerland) · 2023Article
- Calcium Dynamics, WUSCHEL Expression and Callose Deposition during Somatic Embryogenesis inPlants (Basel, Switzerland) · 2023Article
- Calcium dynamics and modulation in carrot somatic embryogenesis.Frontiers in plant science · 2023Article
- Article
- Glutathione and copper ions as critical factors of green plant regeneration efficiency of triticaleFrontiers in plant science · 2022Article
- Embryogenesis of European Radish (Plants (Basel, Switzerland) · 2021Article
- Doubled Haploids in Eggplant.Biology · 2021Review
- Understanding In Vitro Tissue Culture-Induced Variation Phenomenon in Microspore System.International journal of molecular sciences · 2021Review
- Cell Wall Composition and Structure Define the Developmental Fate of Embryogenic Microspores inFrontiers in plant science · 2021Article
- Live Imaging of embryogenic structures in Brassica napus microspore embryo cultures highlights the developmental plasticity of induced totipotent cells.Plant reproduction · 2020Article
- Changes of cell wall components during embryogenesis of Castanea mollissima.Journal of plant research · 2020Article
- Dynamic Changes in Arabinogalactan-Protein, Pectin, Xyloglucan and Xylan Composition of the Cell Wall During Microspore Embryogenesis inFrontiers in plant science · 2019Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microspore embryogenesis is an experimental morphogenic pathway with important applications in basic research and applied plant breeding, but its genetic, cellular, and molecular bases are poorly understood. We applied a multidisciplinary approach using confocal and electron microscopy, detection of Ca2+, callose, and cellulose, treatments with caffeine, digitonin, and endosidin7, morphometry, qPCR, osmometry, and viability assays in order to study the dynamics of cell wall formation during embryogenesis induction in a high-response rapeseed (Brassica napus) line and two recalcitrant rapeseed and eggplant (Solanum melongena) lines. Formation of a callose-rich subintinal layer (SL) was common to microspore embryogenesis in the different genotypes. However, this process was directly related to embryogenic response, being greater in high-response genotypes. A link could be established between Ca2+ influx, abnormal callose/cellulose deposition, and the genotype-specific embryogenic competence. Callose deposition in inner walls and SLs are independent processes, regulated by different callose synthases. Viability and control of internal osmolality are also related to SL formation. In summary, we identified one of the causes of recalcitrance to embryogenesis induction: a reduced or absent protective SL. In responding genotypes, SLs are markers for changes in cell fate and serve as osmoprotective barriers to increase viability in imbalanced in vitro environments. Genotype-specific differences relate to different responses against abiotic (heat/osmotic) stresses.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.