ArticleCell proliferation2019
3-dimensional visualization of implant-tissue interface with the polyethylene glycol associated solvent system tissue clearing method.
Article in Cell proliferation, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 27 citations in OpenAlex.
- In Vitro and In Vivo Evaluation of Yttria-Niobia-Stabilized Zirconia Sandblasted Surface at Bone-Implant Interface with 3-Dimensional Visualization via Advanced Hard Tissue Clearing.Biomaterials research · 2026Article
- Current progress of labeling strategies in tissue clearing for large-scale biological visualization.Frontiers in cell and developmental biology · 2026Review
- Deep imaging of LepRBone research · 2025Article
- Semi-automated protocol to quantify and characterize fluorescent three-dimensional vascular images.PloS one · 2024Article
- Spatiotemporal monitoring of hard tissue development reveals unknown features of tooth and bone development.Science advances · 2023Article
- Label-free cleared tissue microscopy and machine learning for 3D histopathology of biomaterial implants.Journal of biomedical materials research. Part A · 2023Article
- Review
- Alveolar Bone Marrow Gli1+ Stem Cells Support Implant Osseointegration.Journal of dental research · 2022Article
- Knockdown of NRSF Alleviates Ischemic Brain Injury and Microvasculature Defects in Diabetic MCAO Mice.Frontiers in neurology · 2022Article
- Can Developments in Tissue Optical Clearing Aid Super-Resolution Microscopy Imaging?International journal of molecular sciences · 2021Review
- Physical and chemical mechanisms of tissue optical clearing.iScience · 2021Review
- Tissue clearing technique: Recent progress and biomedical applications.Journal of anatomy · 2021Review
- Tissue Clearing and 3-D Visualization of Vasculature with the PEGASOS Method.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Axin2Journal of dental research · 2019Article
- Investigation of Postnatal Craniofacial Bone Development with Tissue Clearing-Based Three-Dimensional Imaging.Stem cells and development · 2019Article
- Tissue Clearing and Its Application to Bone and Dental Tissues.Journal of dental research · 2019Review
- 3-dimensional visualization of implant-tissue interface with the polyethylene glycol associated solvent system tissue clearing method.Cell proliferation · 2019Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
Abstract
objectivesDental implants are major treatment options for restoring teeth loss. Biological processes at the implant-tissue interface are critical for implant osseointegration. Superior mechanical properties of the implant constitute a major challenge for traditional histological techniques. It is imperative to develop new technique to investigate the implant-tissue interface. MATERIALS AND
methodsOur laboratory developed the polyethylene glycol (PEG)-associated solvent system (PEGASOS) tissue clearing method. By immersing samples into various chemical substances, bones and teeth could be turned to transparent with intact internal structures and endogenous fluorescence being preserved. We combined the PEGASOS tissue clearing method with transgenic mouse line and other labelling technique to investigate the angiogenesis and osteogenesis processes occurring at the implant-bone interface.
resultsClearing treatment turned tissue highly transparent and implant could be directly visualized without sectioning. Implant, soft/hard tissues and fluorescent labels were simultaneously imaged in decalcified or non-decalcified mouse mandible samples without disturbing their interfaces. Multi-channel 3-dimensional image stacks at high resolution were acquired and quantified. The processes of angiogenesis and osteogenesis surrounding titanium or stainless steel implants were investigated.
conclusionsBoth titanium and stainless steel implants support angiogenesis at comparable levels. Successful osseointegration and calcium precipitation occurred only surrounding titanium, but not stainless steel implants. PEGASOS tissue clearing method provides a novel approach for investigating the interface between implants and hard tissue.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.