Evidence map›Paper›PMID 30713165›Full record

ArticleActa crystallographica. Section F, Structural biology communications2019

Penetration of dyes into protein crystals.

Alexander McPherson

Abstract read
In one paragraph

Article in Acta crystallographica. Section F, Structural biology communications, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. A note on the appearance of PEG in macromolecular crystals.Acta crystallographica. Section F, Structural biology communications · 2026
    Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

1 author.

Alexander McPhersonMolecular Biology and Biochemistry, University of California Irvine, 3205 McGaugh Hall, Irvine, CA 92697-3900, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Experiments were carried out on 15 different protein crystals with the objective of estimating the rates of penetration of dye molecules into the crystals. The dyes were in the molecular-weight range 250-1000 Da and the protein crystals were of dimensions of 0.7 mm or greater. Experiments were also conducted on protein crystals grown between glass cover slips (separation 200 µm) that restricted the direction of diffusion. The rate of penetration of dyes into protein crystals depends very much on the degree of association between the dye and protein molecules. Dye penetration was not consistent with pure diffusion when the affinity of the protein for the dye was significant, and this was frequent. Penetration rates were less dependent on factors such as the molecular weight of the dye or the diffusion direction. For weakly interacting protein crystal/dye combinations, penetration was a fair measure of diffusivity and the observed rates were in the range 60-100 µm h

Indexed as

Coloring AgentsCrystallizationCrystallography, X-RayDiffusionHumansModels, MolecularProteinsColoring AgentsProteinscolordiffusion ratesmicroscopypenetration ratesstainstime lapse

Identifiers

PMID30713165
PMCPMC6360441

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.