ArticleAnalytica chimica acta2019
Derivatization enhances analysis of estrogens and their bioactive metabolites in human plasma by liquid chromatography tandem mass spectrometry.
Article in Analytica chimica acta, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 48 citations in OpenAlex.
- 17α-Estradiol: A mildly feminizing estrogen with sex-specific metabolic and lifespan benefits.Ageing research reviews · 2026Review
- Differentiation of steroid isomers via derivatization and electron-activated dissociation tandem mass spectrometry.Analytical and bioanalytical chemistry · 2026Article
- Estriol attenuates visceral adiposity and pulmonary artery smooth muscle cell proliferation via ERα-mediated signalling.European heart journal open · 2026Article
- Determination of Hormonal Growth Promotants in Beef Using Liquid Chromatography-Tandem Mass Spectrometry.Drug testing and analysis · 2025Article
- Development of an accurate and sensitive assay for 2-methoxyestradiol using derivatization and liquid chromatography-tandem mass spectrometry.Practical laboratory medicine · 2025Article
- Estrogen profiling in blood and brain: Effects of season and an aggressive interaction in a songbird.PloS one · 2025Article
- Rapid Analysis of Estrogens in Meat Samples by High Performance Liquid Chromatography with Fluorescence Detection.Journal of fluorescence · 2024Article
- Stable and reproducible MIP-ECL sensors for ultra-sensitive and accurate quantitative detection of Estrone.Frontiers in bioengineering and biotechnology · 2024Article
- Derivatization procedure of estradiol with a combination of MPDNP-F and 4-dimethylaminopyridine to generate product ion containing estradiol-skeleton for reliable determination of its serum/plasma concentrations by LC/ESI-MS/MS.Analytical and bioanalytical chemistry · 2024Article
- Novel detection method to rapidly quantify toxic cucurbitacin inJournal of food science and technology · 2023Article
- Endogenous Diurnal Patterns of Adrenal and Gonadal Hormones During a 24-Hour Constant Routine After Simulated Shift Work.Journal of the Endocrine Society · 2022Article
- Profiling acidic metabolites by capillary electrophoresis-mass spectrometry in low numbers of mammalian cells using a novel chemical derivatization approach.Analytical science advances · 2022Article
- Cold-induced phase separation for the simple and reliable extraction of sex hormones for subsequent LC-MS/MS analysis.Journal of lipid research · 2022Article
- Penetrance of Severe Pulmonary Arterial Hypertension in Response to Vascular Endothelial Growth Factor Receptor 2 Blockade in a Genetically Prone Rat Model Is Reduced by Female Sex.Journal of the American Heart Association · 2021Article
- Magnetic Micro-Solid-Phase Extraction Using a Novel Carbon-Based Composite Coupled with HPLC-MS/MS for Steroid Multiclass Determination in Human Plasma.Molecules (Basel, Switzerland) · 2021Article
- Estrogens and Glucocorticoids in Mammary Adipose Tissue: Relationships with Body Mass Index and Breast Cancer Features.The Journal of clinical endocrinology and metabolism · 2020Article
- Simultaneous quantification of estrogens and glucocorticoids in human adipose tissue by liquid-chromatography-tandem mass spectrometry.The Journal of steroid biochemistry and molecular biology · 2019Article
- Quantification of Dehydroepiandrosterone, 17β-Estradiol, Testosterone, and Their Sulfates in Mouse Tissues by LC-MS/MS.Analytical chemistry · 2019Article
- Fulvestrant for the Treatment of Pulmonary Arterial Hypertension.Annals of the American Thoracic Society · 2019Article
- Current strategies for quantification of estrogens in clinical research.The Journal of steroid biochemistry and molecular biology · 2019Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Estrogens regulate many diverse biological processes in health and disease. They circulate at a wide range of concentrations in females generating several active metabolites (hydroxy and methoxyestrogens). The metabolites are assumed to be present in much lower levels and are thought to contribute to diseases such as pulmonary arterial hypertension (PAH). Estrogen metabolites are challenging to quantify in plasma and currently available immunoassays are non-specific. Here we have developed and validated a novel assay to simultaneously quantify parent estrogens and their metabolites by mass spectrometry (MS). Estrogens were extracted from human plasma using solid phase extraction and derivatized using 1-(5-fluoro-2, 4-dinitrophenyl)-4-methylpiperazine (PPZ) before quaternization by methylation ("MPPZ"). MPPZ derivatives were separated and quantified by liquid chromatography tandem MS (LC-MS/MS) in positive electrospray ionization mode, using a QTrap 6500 + coupled to a Shimadzu Nexera X2. Separation was achieved using an ACE Excel 2 C18-PFP column (2 μm, 2.1 mm × 150 mm). The limits of quantification (LOQ) were 0.43-2.17 pg on column with a linear range from 2 or 10 - 2000 pg mL
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.