ArticleActa biomaterialia2019
Patterning of human epidermal stem cells on undulating elastomer substrates reflects differences in cell stiffness.
Article in Acta biomaterialia, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 53 citations in OpenAlex.
- Novel dermis niche equivalent based on a 3D-structure fabricated by 2-photon polymerization.Biomaterials and biosystems · 2026Article
- Non-invasive characterization of the relationship between skin microrelief and dermal-epidermal junction topography using line-field confocal optical coherence tomography (LC-OCT).Scientific reports · 2026Article
- Micropillar Topography Regulates Morphology and Melanogenesis in Melanoma Cells.Journal of functional biomaterials · 2026Article
- Wound healing improvement by a multicomponent wound dressing of keratinocyte-imprinted polydimethylsiloxane substrate in a rabbit model.Scientific reports · 2025Article
- Cell volume regulates terminal differentiation of cultured human epidermal keratinocytes.Journal of cell science · 2025Article
- Mechanochemical bistability of intestinal organoids enables robust morphogenesis.Nature physics · 2025Article
- Telomeres: an organized string linking plants and mammals.Biology direct · 2024Review
- Patterning in stratified epithelia depends on cell-cell adhesion.Life science alliance · 2024Article
- A Novel Method for Fabricating the Undulating Structures at Dermal-Epidermal Junction by Composite Molding Process.Journal of functional biomaterials · 2024Article
- Natural Compounds and Biomimetic Engineering to Influence Fibroblast Behavior in Wound Healing.International journal of molecular sciences · 2024Review
- Myc-dependent dedifferentiation of Gata6Nature cell biology · 2023Article
- Matricellular Proteins in the Homeostasis, Regeneration, and Aging of Skin.International journal of molecular sciences · 2023Review
- Laser Micropatterning Promotes Rete Ridge Formation and Enhanced Engineered Skin Strength without Increased Inflammation.Bioengineering (Basel, Switzerland) · 2023Article
- Recent Tissue Engineering Approaches to Mimicking the Extracellular Matrix Structure for Skin Regeneration.Biomimetics (Basel, Switzerland) · 2023Review
- Understanding Human Epidermal Stem Cells at Single-Cell Resolution.The Journal of investigative dermatology · 2022Review
- Synthesis of Poly(acrylic acid)-Cysteine-Based Hydrogels with Highly Customizable Mechanical Properties for Advanced Cell Culture Applications.ACS omega · 2022Article
- The Importance of Mimicking Dermal-Epidermal Junction for Skin Tissue Engineering: A Review.Bioengineering (Basel, Switzerland) · 2021Review
- A designer cell culture insert with a nanofibrous membrane toward engineering an epithelial tissue model validated by cellular nanomechanics.Nanoscale advances · 2021Article
- Epidermal stem cells maintain stemness via a biomimetic micro/nanofiber scaffold that promotes wound healing by activating the Notch signaling pathway.Stem cell research & therapy · 2021Article
- Mammalian Epidermis: A Compendium of Lipid Functionality.Frontiers in physiology · 2021Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
In human skin the junction between epidermis and dermis undulates, the width and depth of the undulations varying with age and disease. When primary human epidermal keratinocytes are seeded on collagen-coated polydimethylsiloxane (PDMS) elastomer substrates that mimic the epidermal-dermal interface, the stem cells become patterned by 24 h, resembling their organisation in living skin. We found that cell density and nuclear height were higher at the base than the tips of the PDMS features. Cells on the tips not only expressed higher levels of the stem cell marker β1 integrin but also had elevated E-cadherin, Desmoglein 3 and F-actin than cells at the base. In contrast, levels of the transcriptional cofactor MAL were higher at the base. AFM measurements established that the Young's modulus of cells on the tips was lower than on the base or cells on flat substrates. The differences in cell stiffness were dependent on Rho kinase activity and intercellular adhesion. On flat substrates the Young's modulus of calcium-dependent intercellular junctions was higher than that of the cell body, again dependent on Rho kinase. Cell patterning was influenced by the angle of the slope on undulating substrates. Our observations are consistent with the concept that epidermal stem cell patterning is dependent on mechanical forces exerted at intercellular junctions in response to undulations in the epidermal-dermal interface. STATEMENT OF SIGNIFICANCE: In human skin the epidermal-dermal junction undulates and epidermal stem cells are patterned according to their position. We previously created collagen-coated polydimethylsiloxane (PDMS) elastomer substrates that mimic the undulations and provide sufficient topographical information for stem cells to cluster on the tips. Here we show that the stiffness of cells on the tips is lower than cells on the base. The differences in cell stiffness depend on Rho kinase activity and intercellular adhesion. We propose that epidermal stem cell patterning is determined by mechanical forces exerted at intercellular junctions in response to the slope of the undulations.
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