ArticleJournal of assisted reproduction and genetics2019
An integrated investigation of oocyte developmental competence: expression of key genes in human cumulus cells, morphokinetics of early divisions, blastulation, and euploidy.
Article in Journal of assisted reproduction and genetics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 5 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 5 syntheses or guidelines pooled it, 43 citations in OpenAlex.
- Biomarker candidates for oocyte and embryo quality assessment in assisted reproduction: A systematic review.JBRA assisted reproduction · 2025Pooled it
- The Functional Role of Cumulus Cells and Their Influence on Oocyte Quality: A Systematic Review.Reproductive sciences (Thousand Oaks, Calif.) · 2025Pooled it
- Biomarkers Assessing the Role of Cumulus Cells on IVF Outcomes: A Systematic Review.Journal of assisted reproduction and genetics · 2024Pooled it
- Unravelling the role of HAS2, GREM1, and PTGS2 gene expression in cumulus cells: implications for human oocyte development competency - a systematic review and integrated bioinformatic analysis.Frontiers in endocrinology · 2024Pooled it
- Effect of the time interval between oocyte retrieval and ICSI on embryo development and reproductive outcomes: a systematic review.Reproductive biology and endocrinology : RB&E · 2021Pooled it
- Improved clinical outcomes after non-invasive oocyte selection and Day 3 eSET in ICSI patients.Reproductive biology and endocrinology : RB&E · 2021Trial
- Proprotein convertase activity regulates cumulus-oocyte-complex matrix integrity and cumulus cell migration during ovulation via a GDF9-dependent mechanism.bioRxiv : the preprint server for biology · 2026Article
- Prediction of high-quality human oocytes and embryos through the analysis of gene expression in cumulus cells.Journal of ovarian research · 2025Review
- [Role of hyaluronic acid synthases in female infertility-related diseases].Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences · 2025Review
- Endometriosis-associated infertility alters the microRNA signatures of cumulus cells with a particularly pronounced effect in oocytes that failed fertilization.Biological research · 2025Article
- Artificial intelligence outperforms humans in morphology-based oocyte selection in cattle.Scientific reports · 2025Article
- Changes in Gene Transcription in Cumulus Cells from Women of Advanced Maternal Age under the Influence of Extracellular Vesicles from Follicular Fluid of Young Donors.Bulletin of experimental biology and medicine · 2025Article
- Maturational competence of equine oocytes is associated with alterations in their 'cumulome'.Molecular human reproduction · 2024Article
- Extracellular vesicles secreted by cumulus cells contain microRNAs that are potential regulatory factors of mouse oocyte developmental competence.Molecular human reproduction · 2024Article
- A novel machine-learning framework based on early embryo morphokinetics identifies a feature signature associated with blastocyst development.Journal of ovarian research · 2024Article
- When the Embryo Meets the Endometrium: Identifying the Features Required for Successful Embryo Implantation.International journal of molecular sciences · 2024Review
- Using Cumulus Cell Biopsy as a Non-Invasive Tool to Access the Quality of Bovine Oocytes: How Informative Are They?Animals : an open access journal from MDPI · 2022Article
- TEffect of Single Embryo Blastomere Biopsy from Human Frozen Embryos on Assisted Reproductive Outcomes.Cell journal · 2022Article
- GPX3 Overexpression in Cumulus Cells Entails a Poor Prognosis for Uterine Implantation of Morphotype A Embryos.Biology · 2022Article
- Effects of Poly (ADP-ribose) Polymerase Inhibition on DNA Integrity and Gene Expression in Ovarian Follicular Cells in Mice with EndotoxemiaIranian biomedical journal · 2022Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeTo investigate the association of cumulus cell (CC)-related expression of a selected cluster of key genes (PTGS2, CAMK1D, HAS2, STC1, and EFNB2) with embryo development to blastocyst.
methodsExploratory study at a private clinic. Eighteen advanced maternal age patients were enrolled (37.3 ± 4.0 years). Seventy-five cumuli were collected, whose oocytes resulted in either developmental arrest (N = 33) or blastocyst formation (N = 42). The noninvasive CC gene expression was combined with time-lapse morphokinetic parameters and, for blastocysts, with qPCR-based aneuploidy testing on trophectoderm biopsies.
resultsThe detection rate was 100% for all transcripts, but STC1 (96%) and CAMK1D (89%). Among amplified assays, CC mean expression levels of CAMK1D, PTGS2, and HAS2 were higher from oocytes that developed to blastocyst. No difference in CC key gene expression was reported between euploid (N = 21) and aneuploid (N = 21) blastocysts. Some timings of early embryo development were faster in embryos developing to blastocyst (time of pronuclei appearance and fading, division to two- and four-cells, first and second cell cycles). However, the generalized linear models outlined increasing CAMK1D expression levels as the strongest parameter associated with oocytes' developmental potential from both a general (AUC = 0.78 among amplified samples) and an intrapatient perspectives (AUC = 0.9 among patients obtaining ≥ 2 zygotes from the cohort with different developmental outcomes).
conclusionsCAMK1D level of expression in CCs associated with blastocyst development. If confirmed from larger studies in wider populations of patients, the investigation of CC key gene expression might suit IVF clinics not adopting blastocyst culture. Future investigations should clarify the role of CAMK1D in ovarian physiology and could provide novel insights on how oocytes gain competence during folliculogenesis.
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Registered trials
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