ArticleAnalytical and bioanalytical chemistry2019
Toward an efficient workflow for the analysis of the human milk peptidome.
Article in Analytical and bioanalytical chemistry, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- A Proteomic Atlas of Human Milk: Uncovering Milk Functional Components.Research square · 2026Article
- Article
- Challenges and Opportunities in Quantifying Bioactive Compounds in Human Breastmilk.Biomolecules · 2025Review
- Optimizing workflow efficiency for analyzing low molecular weight endogenous peptides in colostrum.RSC advances · 2024Article
- Network analysis of the proteome and peptidome sheds light on human milk as a biological system.Scientific reports · 2024Article
- Blood-supplementing effect of low molecular weight peptides of E-Jiao on chemotherapy-induced myelosuppression: evaluation of pharmacological activity and identification of bioactive peptides releasedFrontiers in pharmacology · 2024Article
- Isolation and characterization of antioxidant peptides fromFood science & nutrition · 2023Article
- Exploring Human Milk Dynamics: Interindividual Variation in Milk Proteome, Peptidome, and Metabolome.Journal of proteome research · 2022Article
- Monitoring Human Milk β-Casein Phosphorylation andInternational journal of molecular sciences · 2021Article
- A literature review on lactopontin and its roles in early life.Translational pediatrics · 2021Review
- [Effect of sample preparation on analysis of human milk endogenous peptides using liquid chromatography-tandem mass spectrometry].Se pu = Chinese journal of chromatography · 2021Article
- Personalized Profiling Reveals Donor- and Lactation-Specific Trends in the Human Milk Proteome and Peptidome.The Journal of nutrition · 2021Observational
- Human milk and infant formulae: Peptide differences and the opportunity to address the functional gap.Current research in food science · 2020Article
- Distinct expression profiles of peptides in placentae from preeclampsia and normal pregnancies.Scientific reports · 2020Article
- Differences and Similarities in the Peptide Profile of Preterm and Term Mother's Milk, and Preterm and Term Infant Gastric Samples.Nutrients · 2020Article
- The Functional Power of the Human Milk Proteome.Nutrients · 2019Review
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Authors and funding
5 authors.
Funding
Abstract
There is a growing interest for investigating endogenous peptides from human biofluids which may provide yet unknown functional benefits or provide an early indication of disease states as potential biomarkers. A major technical bottleneck in the investigation of endogenous peptides from body fluids, e.g., serum, urine, saliva, and milk, is that each of these fluids seems to require unique workflows for peptide extraction and analysis. Thus, protocols optimized for serum cannot be directly translated to milk. One biofluid that is readily available, but which has not been extensively explored, is human milk, whose analysis could contribute to our understanding of the immune development of the newborn infant. Due to the occurrence of highly abundant lipids, proteins, and saccharides, milk peptidomics requires dedicated sample preparation steps. The aim of this study was to develop a time and cost-efficient workflow for the analysis of the human milk peptidome, for which we compared peptide extraction methodologies and peptide fragmentation methods. A method using strong acid protein precipitation and analysis by collision-induced dissociation fragmentation was found to be superior to all other test methods, allowing us qualitative and quantitative detection of about 4000 endogenous human milk peptides in a total analysis time of just 18 h.
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