ArticleScientific reports2019
Transcriptomic response of primary human airway epithelial cells to flavoring chemicals in electronic cigarettes.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 67 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
67 citing papers in PubMed, 2 syntheses or guidelines pooled it, 110 citations in OpenAlex.
- Advances in Molecular Research of Tracheobronchial Tree Aging: A Systematic Review.International journal of molecular sciences · 2025Pooled it
- Pulmonary effects of e-liquid flavors: a systematic review.Journal of toxicology and environmental health. Part B, Critical reviews · 2022Pooled it
- Cardiopulmonary effects of very low nicotine content cigarettes with and without access to e-cigarettes in vulnerable populations.Preventive medicine · 2025Trial
- Acute respiratory responses to the use of e-cigarette: an intervention study.Scientific reports · 2019Trial
- Impact of e-cigarette vaping on the immune system across the body.Physiological reviews · 2026Review
- Vaping-related lung disease: a narrative review.Journal of thoracic disease · 2026Review
- Methylglyoxal and Glyoxal in High-Puff Disposable Electronic Cigarette Liquids: Unexpected Accumulation and Enhanced Cytotoxicity.ACS omega · 2026Article
- MI-181 enhances ciliation and cilia length in a cigarette smoke exposed airway epithelial model.Scientific reports · 2026Article
- The essential role of cytoskeleton and ciliary abnormalities in the development of congenital pulmonary airway malformations.Pediatric surgery international · 2025Article
- Disposable e-cigarette use: Factors, frequency and cigarette smoking among United States high school students.Addiction (Abingdon, England) · 2025Article
- The rodent electronic nicotine delivery system: Apparatus for voluntary nose-only e-cigarette aerosol inhalation.Journal of the experimental analysis of behavior · 2025Article
- Evaluation of E-Cigarette Effects on Gene Expression Profile of Human Bronchial Epithelial Cells: A System Biology Approach.Tanaffos · 2025Article
- Oxidized phospholipid and transcriptomic signatures of THC-related vaping associated lung injury.Scientific reports · 2024Article
- Toxicity of microplastic fibers containing azobenzene disperse dyes to human lung epithelial cells cultured at an air-liquid interface.Journal of hazardous materials · 2024Article
- Article
- Single-cell transcriptomics reveals e-cigarette vapor-induced airway epithelial remodeling and injury.Respiratory research · 2024Article
- Use of Electronic Nicotine Delivery Systems and Age of Asthma Onset Among US Adults and Youths.JAMA network open · 2024Article
- Single-cell resolution of human airway epithelial cells exposed to bronchiolitis obliterans-associated chemicals.American journal of physiology. Lung cellular and molecular physiology · 2024Article
- Nebulized Menthol Impairs Mucociliary Clearance via TRPM8 and MUC5AC/MUC5B in Primary Airway Epithelial Cells.International journal of molecular sciences · 2023Article
- Impact of the FDA flavour enforcement policy on flavoured electronic cigarette use behaviour changes.Tobacco control · 2022Article
7 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
The widespread use of electronic cigarettes (e-cigarettes or e-cig) is a growing public health concern. Diacetyl and its chemical cousin 2,3-pentanedione are commonly used to add flavors to e-cig; however, little is known about how the flavoring chemicals may impair lung function. Here we report that the flavoring chemicals induce transcriptomic changes and perturb cilia function in the airway epithelium. Using RNA-Seq, we identified a total of 163 and 568 differentially expressed genes in primary normal human bronchial epithelial (NHBE) cells that were exposed to diacetyl and 2,3-pentanedione, respectively. DAVID pathway analysis revealed an enrichment of cellular pathways involved in cytoskeletal and cilia processes among the set of common genes (142 genes) perturbed by both diacetyl and 2,3-pentanedione. Consistent with this, qRT-PCR confirmed that the expression of multiple genes involved in cilia biogenesis was significantly downregulated by diacetyl and 2,3-pentanedione in NHBE cells. Furthermore, immunofluorescence staining showed that the number of ciliated cells was significantly decreased by the flavoring chemicals. Our study indicates that the two widely used e-cig flavoring chemicals impair the cilia function in airway epithelium and likely contribute to the adverse effects of e-cig in the lung.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.