ArticleNature communications2019
Smart scanning for low-illumination and fast RESOLFT nanoscopy in vivo.
Article in Nature communications, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
34 citing papers in PubMed.
- Time-lapse RESOLFT nanoscopy empowered by deep learning.Nature communications · 2026Article
- Smart event-triggered MINFLUX microscopy to catch and follow rare events.Nature communications · 2026Article
- A Versatile Tool to Predict and Guide RESOLFT Images Based on Photoswitching, Labelling and Optical Properties.Chemphyschem : a European journal of chemical physics and physical chemistry · 2026Article
- Review
- Quantitative optical nanoscopy of mitochondrial-derived vesicles in neurons classifies pre-peroxisomal and clearing organelles.Nature communications · 2026Article
- Closed-loop optogenetic control of cell biology enables outcome-driven microscopy.Nature communications · 2025Article
- Adaptive fluorescence lifetime imaging with per-pixel signal optimization and flexible scanning.Biomedical optics express · 2025Article
- Self-driving microscopy detects the onset of protein aggregation and enables intelligent Brillouin imaging.Nature communications · 2025Article
- Advances and future trends in real-time precision optical control of chemical processes in live cells.Npj imaging · 2025Review
- Live-cell imaging powered by computation.Nature reviews. Molecular cell biology · 2024Review
- Imaging actin organisation and dynamics in 3D.Journal of cell science · 2024Article
- Development of AI-assisted microscopy frameworks through realistic simulation with pySTED.Nature machine intelligence · 2024Article
- Light-sheets and smart microscopy, an exciting future is dawning.Communications biology · 2023Review
- Extending fluorescence anisotropy to large complexes using reversibly switchable proteins.Nature biotechnology · 2023Article
- An open-source microscopy framework for simultaneous control of image acquisition, reconstruction, and analysis.HardwareX · 2023Article
- Event-driven acquisition for content-enriched microscopy.Nature methods · 2022Article
- Event-triggered STED imaging.Nature methods · 2022Article
- Genetically encoded photo-switchable molecular sensors for optoacoustic and super-resolution imaging.Nature biotechnology · 2022Article
- Technological advances in super-resolution microscopy to study cellular processes.Molecular cell · 2022Review
- Studying the Dynamics of Chromatin-Binding Proteins in Mammalian Cells Using Single-Molecule Localization Microscopy.Methods in molecular biology (Clifton, N.J.) · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RESOLFT fluorescence nanoscopy can nowadays image details far beyond the diffraction limit. However, signal to noise ratio (SNR) and temporal resolution are still a concern, especially deep inside living cells and organisms. In this work, we developed a non-deterministic scanning approach based on a real-time feedback system which speeds up the acquisition up to 6-fold and decreases the light dose by 70-90% for in vivo imaging. Also, we extended the information content of the images by acquiring the complete temporal evolution of the fluorescence generated by reversible switchable fluorescent proteins. This generates a series of images with different spatial resolution and SNR, from conventional to RESOLFT images, which combined through a multi-image deconvolution algorithm further enhances the effective resolution. We reported nanoscale imaging of organelles up to 35 Hz and actin dynamics during an invasion process at a depth of 20-30 µm inside a living Caenorhabditis elegans worm.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.